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首页> 美国卫生研究院文献>The Scientific World Journal >Comparative Studies on Cellular Behaviour of Carnation (Dianthus caryophyllus Linn. cv. Grenadin) Grown In Vivo and In Vitro for Early Detection of Somaclonal Variation
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Comparative Studies on Cellular Behaviour of Carnation (Dianthus caryophyllus Linn. cv. Grenadin) Grown In Vivo and In Vitro for Early Detection of Somaclonal Variation

机译：康乃馨（Dianthus caryophyllus Linn.cv. Grenadin）体内和体外生长以早期检测体细胞克隆变异的细胞行为的比较研究

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The present study deals with the cytological investigations on the meristematic root cells of carnation (Dianthus caryophyllus Linn.) grown in vivo and in vitro. Cellular parameters including the mitotic index (MI), chromosome count, ploidy level (nuclear DNA content), mean cell and nuclear areas, and cell doubling time (Cdt) were determined from the 2 mm root tip segments of this species. The MI value decreased when cells were transferred from in vivo to in vitro conditions, perhaps due to early adaptations of the cells to the in vitro environment. The mean chromosome number was generally stable (2n = 2x = 30) throughout the 6-month culture period, indicating no occurrence of early somaclonal variation. Following the transfer to the in vitro environment, a significant increase was recorded for mean cell and nuclear areas, from 26.59 ± 0.09 μm2 to 35.66 ± 0.10 μm2 and 142.90 ± 0.59 μm2 to 165.05 ± 0.58 μm2, respectively. However, the mean cell and nuclear areas of in vitro grown D. caryophyllus were unstable and fluctuated throughout the tissue culture period, possibly due to organogenesis or rhizogenesis. Ploidy level analysis revealed that D. caryophyllus root cells contained high percentage of polyploid cells when grown in vivo and maintained high throughout the 6-month culture period.
机译：本研究涉及在体内和体外生长的康乃馨（Dianthus caryophyllus Linn。）的分生组织根细胞的细胞学研究。细胞参数包括有丝分裂指数（MI），染色体数，倍性水平（核DNA含量），平均细胞和核面积以及细胞倍增时间（Cdt），是从该物种的2 mm根尖片段确定的。当细胞从体内转移到体外条件下时，MI值降低了，这可能是由于细胞对体外环境的早期适应所致。在整个6个月的培养期内，平均染色体数通常是稳定的（2n = 2x = 30），这表明没有早期体细胞克隆变异发生。转移到体外环境后，记录的平均细胞和核面积显着增加，从26.​​59±0.09μm 2 增至35.66±0.10μm 2 和142.90± 0.59μm 2 分别为165.05±0.58μmμ m 2 。然而，体外生长的 D的平均细胞和核面积。在整个组织培养期间，石竹都是不稳定的，并且可能是由于器官发生或发根而波动的。倍性水平分析表明， D。体内生长时，石竹根细胞含有较高比例的多倍体细胞，并且在整个6个月的培养期间均保持较高水平。

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