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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >The Evolution of the Bacterial Luciferase Gene Cassette (lux) as a Real-Time Bioreporter
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The Evolution of the Bacterial Luciferase Gene Cassette (lux) as a Real-Time Bioreporter

机译：荧光素酶基因盒（lux）作为实时生物报告的演变。

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The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last 30 years to include expression in eukaryotic cells such as Saccharomyces cerevisiae and even human cell lines as well. Under these conditions, the lux system has been developed for use as a biomedical detection tool for toxicity screening and visualization of tumors in small animal models. As the technologies for lux signal detection continue to improve, it is poised to become one of the first fully implantable detection systems for intra-organismal optical detection through direct marriage to an implantable photon-detecting digital chip. This review presents the basic biochemical background that allows the lux system to continuously autobioluminesce and highlights the important milestones in the use of lux-based bioreporters as they have evolved from chemical detection platforms in prokaryotic bacteria to rodent-based tumorigenesis study targets. In addition, the future of lux imaging using integrated circuit microluminometry to image directly within a living host in real-time will be introduced and its role in the development of dose/response therapeutic systems will be highlighted.
机译：细菌荧光素酶基因盒（lux）在生物发光生物报告系统中是独一无二的，因为它具有合成和/或清除产生光所需的所有底物化合物的能力。因此，勒克斯系统具有独特的能力，可以在各种各样的生物宿主中连续或响应特定触发条件而自主产生发光信号。勒克斯最初广泛用作细菌生物报告系统，用于检测环境样品中的特定化学信号，但在过去30年中，勒克斯作为生物报告技术的应用一直在不断扩展，包括在真核细胞（如酿酒酵母）甚至人细胞中表达行。在这些条件下，已经开发出lux系统用作生物医学检测工具，用于在小动物模型中进行毒性筛查和肿瘤可视化。随着勒克斯信号检测技术的不断改进，通过直接与可植入光子检测数字芯片结合，它有望成为用于生物体内光学检测的首批完全可植入检测系统之一。这篇综述介绍了使lux系统连续进行生物发光的基本生化背景，并强调了基于lux的生物报道基因的使用中的重要里程碑，因为它们已经从原核细菌的化学检测平台发展成为基于啮齿动物的肿瘤发生研究目标。此外，将介绍使用集成电路微光度法直接在活体宿主内实时成像的勒克斯成像的未来，并将突出其在剂量/反应治疗系统开发中的作用。

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