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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >A PDMS-Based Cylindrical Hybrid Lens for Enhanced Fluorescence Detection in Microfluidic Systems
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A PDMS-Based Cylindrical Hybrid Lens for Enhanced Fluorescence Detection in Microfluidic Systems

机译：基于PDMS的圆柱混合透镜用于微流控系统中增强的荧光检测

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Microfluidic systems based on fluorescence detection have been developed and applied for many biological and chemical applications. Because of the tiny amount of sample in the system; the induced fluorescence can be weak. Therefore, most microfluidic systems deploy multiple optical components or sophisticated equipment to enhance the efficiency of fluorescence detection. However, these strategies encounter common issues of complex manufacturing processes and high costs. In this study; a miniature, cylindrical and hybrid lens made of polydimethylsiloxane (PDMS) to improve the fluorescence detection in microfluidic systems is proposed. The hybrid lens integrates a laser focusing lens and a fluorescence collecting lens to achieve dual functions and simplify optical setup. Moreover, PDMS has advantages of low-cost and straightforward fabrication compared with conventional optical components. The performance of the proposed lens is first examined with two fluorescent dyes and the results show that the lens provides satisfactory enhancement for fluorescence detection of Rhodamine 6G and Nile Red. The overall increments in collected fluorescence signal and detection sensitivity are more than 220% of those without lens, and the detection limits of Rhodamine 6G and Nile red are lowered to 0.01 μg/mL and 0.05 μg/mL, respectively. The hybrid lens is further applied to the detection of Nile red-labeled Chlorella vulgaris cells and it increases both signal intensity and detection sensitivity by more than 520%. The proposed hybrid lens also dramatically reduces the variation in detected signal caused by the deviation in incident angle of excitation light.
机译：已经开发了基于荧光检测的微流体系统，并将其应用于许多生物和化学应用。由于系统中的样品量很少；诱导的荧光可能很弱。因此，大多数微流体系统部署了多个光学组件或复杂的设备，以提高荧光检测的效率。但是，这些策略遇到了复杂的制造过程和高成本的常见问题。在这个研究中;提出了一种由聚二甲基硅氧烷（PDMS）制成的微型，圆柱形和混合透镜，以改善微流控系统中的荧光检测。混合透镜集成了激光聚焦透镜和荧光收集透镜，以实现双重功能并简化光学设置。此外，与传统的光学组件相比，PDMS具有低成本和直接制造的优势。首先用两种荧光染料检查了所提议镜片的性能，结果表明该镜片为罗丹明6G和尼罗红的荧光检测提供了令人满意的增强。所收集的荧光信号和检测灵敏度的总增量比不使用透镜的总增量高220％，若丹明6G和尼罗红的检测限分别降低至0.01μg/ mL和0.05μg/ mL。混合透镜进一步应用于尼罗河红标记的小球藻细胞的检测，它将信号强度和检测灵敏度提高了520％以上。所提出的混合透镜还极大地减小了由激发光的入射角的偏差引起的检测信号的变化。

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