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First draft genome sequence of a strain from the genus Fusibacter isolated from Salar de Ascotán in Northern Chile

机译:来自智利北部撒拉族德斯科特(Salar deAscotán)分离的Fusibacter菌株的基因组序列初稿

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摘要

Fusibacter sp. 3D3 (ATCC BAA-2418) is an arsenate-reducing halotolerant strain within the Firmicutes phylum, isolated from the Salar de Ascotán, a hypersaline salt flat in Northern Chile. This high-Andean closed basin is an athalassohaline environment located at the bottom of a tectonic basin surrounded by mountain range, including some active volcanoes. This landscape can be an advantageous system to explore the effect of salinity on microorganisms that mediate biogeochemical reactions. Since 2000, microbial reduction of arsenic has been evidenced in the system, and the phylogenetic analysis of the original community plus the culture enrichments has revealed the predominance of Firmicutes phylum. Here, we describe the first whole draft genome sequence of an arsenic-reducing strain belonging to the Fusibacter genus showing the highest 16S rRNA gene sequence similarity (98%) with Fusibacter sp. strain Vns02. The draft genome consists of 57 contigs with 5,111,250 bp and an average G + C content of 37.6%. Out of 4780 total genes predicted, 4700 genes code for proteins and 80 genes for RNAs. Insights from the genome sequence and some microbiological features of the strain 3D3 are available under Bioproject accession PRJDB4973 and Biosample SAMD00055724. The release of the genome sequence of this strain could contribute to the understanding of the arsenic biogeochemistry in extreme environments.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-017-0252-4) contains supplementary material, which is available to authorized users.
机译:镰刀菌3D3(ATCC BAA-2418)是Firmicutes门上的一种降低砷酸盐的耐盐菌,从智利北部的高盐盐沼Salar deAscotán分离出来。这个高安第斯山脉的封闭盆地是一个邻卤代盐环境,位于一个被山脉环绕的构造盆地的底部,包括一些活火山。这种景观可能是探索盐度对介导生物地球化学反应的微生物的影响的有利系统。自2000年以来,该系统已证明了微生物对砷的还原作用,对原始群落和培养物富集的系统发育分析表明,该种是优势种。在这里,我们描述了属于Fusibacter属的减少砷的菌株的第一个完整的基因组序列,该序列显示了与Fusibacter sp。最高的16S rRNA基因序列相似性(98%)。菌株Vns02。基因组草案由57个重叠群组成,共有5,111,250 bp,平均G + C含量为37.6%。在总共预测的4780个基因中,有4700个基因编码蛋白质,而80个基因编码RNA。可从Bioproject登录号PRJDB4973和Biosample SAMD00055724获得有关3D3菌株的基因组序列和某些微生物学特征的见解。该菌株的基因组序列的释放可能有助于理解极端环境中的砷生物地球化学。电子补充材料本文的在线版本(doi:10.1186 / s40793-017-0252-4)包含补充材料,可以通过以下途径获得给授权用户。

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