Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

机译：果蝇天线中嗅觉受体神经元的整装免疫标记

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Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this information to the brain. As such, determining how olfactory information is transferred to the brain, modifying both perception and behavior, merits investigation. Interestingly, there is emerging evidence that cellular transduction and transcriptional factors are involved in the diversification of olfactory receptor neuron. Here we provide a robust whole mount immunological labeling method to assay in vivo olfactory receptor neuron organization. Using this method, we identified all olfactory receptor neurons with anti-ELAV antibody, a known pan-neural marker and Or49a-mCD8::GFP, an olfactory receptor neuron specifically expressed in Nba neuron using anti-GFP antibody.

机译：气味分子以精确和协调的方式结合其靶标受体。每个受体识别一个特定的信号，并将该信息传递给大脑。因此，确定嗅觉信息如何传递到大脑，修改感知和行为都值得进行研究。有趣的是，越来越多的证据表明，细胞转导和转录因子参与了嗅觉受体神经元的多样化。在这里，我们提供了一种强大的整体安装免疫标记方法来测定体内嗅觉受体神经元组织。使用这种方法，我们用抗ELAV抗体，已知的泛神经标记物和Or49a-mCD8 :: GFP（使用抗GFP抗体在Nba神经元中特异性表达的嗅觉受体神经元）鉴定了所有嗅觉受体神经元。

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期刊名称 Journal of Visualized Experiments : JoVE
作者
M. Rezaul Karim; Keita Endo; Adrian W Moore; Hiroaki Taniguchi;
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作者单位

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年(卷),期 2014(-1),87
年度 2014
页码 51245
总页数 4
原文格式 PDF
正文语种
中图分类眼科学;
关键词
Neuroscience Issue 87 Developmental biology Drosophila Whole mount immunolabeling olfactory receptor neurons antennae sensory organ;

机译：神经科学;第87期;发育生物学;果蝇;完整安装的免疫标记;嗅觉受体神经元;触角;感觉器官;

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专利

1. Orthodenticle is required for the development of olfactory projection neurons and local interneurons in Drosophila Orthodenticle is required for the development of olfactory projection neurons and local interneurons in Drosophila Orthodenticle is required for the development of olfactory projection neurons and local interneurons in Drosophila [J] . K. VijayRaghavan, Sonia Sen, Heinrich Reichert, Biology Open . 2014,第8期

机译：在果蝇中，嗅觉投射神经元和局部中间神经元的发育需要齿科。在果蝇中，嗅觉投射神经元和局部中间神经元的发育需要齿科
2. Functional and molecular evolution of olfactory neurons and receptors for aliphatic esters across the Drosophila genus [J] . de Bruyne M., Smart R., Zammit E., Journal of Comparative Physiology, A. Sensory, Neural, and Behavioral Physiology . 2010,第2期

机译：果蝇属中嗅觉神经元和脂族酯受体的功能和分子进化
3. Functional and molecular evolution of olfactory neurons and receptors for aliphatic esters across the Drosophila genus [J] . Marien de Bruyne, Renee Smart, Elizabeth Zammit, Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology . 2010,第2期

机译：果蝇属中嗅觉神经元和脂族酯受体的功能和分子进化
4. Improving odorant chemical class prediction with multi-layer perceptrons using temporal odorant spike responses from drosophila melanogaster olfactory receptor neurons [C] . Luqman R. Bachtiar, Richard D. Newcomb, Andrew V. Kralicek, Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2016

机译：使用果蝇嗅觉受体神经元的瞬时气味尖峰响应，通过多层感知器改善气味化学类别的预测
5. Starved State Dependent Modulation of Olfactory Receptor Neuron Function in Drosophila melanogaster Larvae [D] . Kollala, Sai Sundeep. 2017

机译：果蝇果蝇嗅觉神经元功能的饥饿状态依赖调节。
6. Variant Ionotropic Receptors Are Expressed in Olfactory Sensory Neurons of Coeloconic Sensilla on the Antenna of the Desert Locust (Schistocerca gregaria) [O] . Mei Guo, Jürgen Krieger, Ewald Große-Wilde, 2014

机译：在沙漠蝗虫（Schistocerca gregaria）天线上的腔锥感官的嗅觉感觉神经元中表达不同的离子受体。
7. Variant ionotropic receptors are expressed in olfactory sensory neurons of coeloconic sensilla on the antenna of the desert locust (Schistocerca gregaria) [O] . Guo, M., Krieger, J., Grosse-Wilde, E., 2014

机译：变异离子型受体在沙漠蝗虫（schistocerca gregaria）天线上的coeloconic sensilla的嗅觉感觉神经元中表达

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

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