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首页> 美国卫生研究院文献>Theranostics >Rapid and Ultrasensitive Quantification of Multiplex Respiratory Tract Infection Pathogen via Lateral Flow Microarray based on SERS Nanotags
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Rapid and Ultrasensitive Quantification of Multiplex Respiratory Tract Infection Pathogen via Lateral Flow Microarray based on SERS Nanotags

机译：通过基于SERS纳米标签的侧向流微阵列快速和超灵敏定量多重呼吸道感染病原体

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Respiratory tract infections (RTIs) are severe acute infectious diseases, which require the timely and accurate identification of the pathogens involved so that the individual treatment plan can be selected, including optimized use of antibiotics. However, high throughput and ultrasensitive quantification of multiple nucleic acids is a challenge in a point of care testing (POCT) device.>Methods: Herein, we developed a 2×3 microarray on a lateral flow strip with surface enhanced Raman scattering (SERS) nanotags encoding the nucleic acids of 11 common RTI pathogens. On account of the signal magnification of encoded SERS nanotags in addition to the high surface area to volume ratio of the nitrocellulose (NC) membrane, rapid quantification of the 11 pathogens with a broad linear dynamic range (LDR) and ultra-high sensitivity was achieved on one lateral flow microarray.>Results: The limit of detection (LOD) for influenza A, parainfluenza 1, parainfluenza 3, respiratory syncytial virus, coxiella burnetii, legionella pneumophila, influenza B, parainfluenza 2, adenovirus, chlamydophila pneumoniae, and mycoplasma pneumoniae were calculated to be 0.031 pM, 0.030 pM, 0.038 pM, 0.038 pM, 0.040 pM, 0.039 pM, 0.035 pM, 0.032 pM, 0.040 pM, 0.039 pM, and 0.041 pM, respectively. The LDR of measurement of the target nucleic acids of the eleven RTI pathogens were 1 pM-50 nM, which span 5 orders of magnitude.>Conclusions: We anticipate this novel approach could be widely adopted in the early and precise diagnosis of RTI and other diseases.
机译：呼吸道感染（RTIs）是严重的急性传染病，需要及时，准确地确定所涉及的病原体，以便可以选择单独的治疗计划，包括优化使用抗生素。但是，高通量和多种核酸的超灵敏定量是即时检测（POCT）设备的一个挑战。>方法：本文中，我们在带有表面的侧向流动条上开发了2×3微阵列增强的拉曼散射（SERS）纳米标签编码11种常见RTI病原体的核酸。除了硝化纤维素（NC）膜的高表面积与体积比外，由于编码的SERS纳米标签的信号放大，还可以快速定量测定11种病原体，并具有宽线性动态范围（LDR）和超高灵敏度>结果：甲型流感，副流感1，副流感3，呼吸道合胞病毒，伯氏柯氏杆菌，嗜肺军团菌，乙型流感，副流感2，腺病毒，肺炎衣原体和肺炎支原体分别计算为0.031 pM，0.030 pM，0.038 pM，0.038 pM，0.040 pM，0.039 pM，0.035 pM，0.032 pM，0.040 pM，0.039 pM和0.041 pM。对11种RTI病原体的靶核酸进行测量的LDR为1 pM-50 nM，跨度为5个数量级。>结论：我们希望这种新方法可在早期和晚期广泛采用。精确诊断RTI等疾病。

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