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Analysis of Deoxynivalenol and Deoxynivalenol-3-glucoside in Hard Red Spring Wheat Inoculated with Fusarium Graminearum

机译:小麦赤霉病菌侵染的硬红春小麦中脱氧雪茄烯醇和脱氧雪茄烯醇-3-葡萄糖苷的分析

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摘要

Deoxynivalenol (DON) is a mycotoxin affecting wheat quality. The formation of the “masked” mycotoxin deoxinyvalenol-3-glucoside (D3G) results from a defense mechanism the plant uses for detoxification. Both mycotoxins are important from a food safety point of view. The aim of this work was to analyze DON and D3G content in inoculated near-isogenic wheat lines grown at two locations in Minnesota, USA during three different years. Regression analysis showed positive correlation between DON content measured with LC and GC among wheat lines, locality and year. The relationship between DON and D3G showed a linear increase until a certain point, after which the DON content and the D3G increased. Wheat lines having higher susceptibility to Fusarium showed the opposite trend. ANOVA demonstrated that the line and location have a greater effect on variation of DON and D3G than do their interaction among years. The most important factor affecting DON and D3G was the growing location. In conclusion, the year, environmental conditions and location have an effect on the D3G/DON ratio in response to Fusarium infection.
机译:脱氧雪腐酚(DON)是一种影响小麦品质的霉菌毒素。 “被掩盖的”霉菌毒素脱氧新戊烯醇-3-葡萄糖苷(D3G)的形成是由植物用于排毒的防御机制导致的。从食品安全的角度来看,两种霉菌毒素都很重要。这项工作的目的是分析在美国明尼苏达州三个不同年份种植的近等基因接种小麦品系中的DON和D3G含量。回归分析表明,用LC和GC测得的DON含量在小麦品系,产地和年份之间呈正相关。 DON和D3G之间的关系直到某个点呈线性增加,此后DON含量和D3G增加。对镰刀菌敏感性较高的小麦品系表现出相反的趋势。方差分析表明,线和位置对DON和D3G的变化的影响要大于它们之间的相互作用。影响DON和D3G的最重要因素是位置的增长。总之,年份,环境条件和位置对镰刀菌感染的D3G / DON比值有影响。

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