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首页> 美国卫生研究院文献>Tropical Life Sciences Research >Isolation and Characterisation of Endophytic Nitrogen Fixing Bacteria in Sugarcane
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Isolation and Characterisation of Endophytic Nitrogen Fixing Bacteria in Sugarcane

机译：甘蔗内生固氮菌的分离与鉴定

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摘要

Endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. U-Thong 3; UT3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (N)-free sand. Eighty nine isolates of endophytic bacteria were obtained on N-free agar. An acetylene reduction assay (ARA) detected nitrogenase activity in all 89 isolates. Three isolates from the chewing (C2HL2, C7HL1 and C34MR1) sugarcane and one isolate from the industrial sugarcane (UT3R1) varieties were characterised, and their responses to different yeast extract concentrations were investigated. Three different responses in nitrogenase activity were observed. Isolates C2HL2 and C7HL1 exhibited major increases with the addition of 0.005% yeast extract, C34MR1 exhibited no response, and UT3R1 exhibited a significant decrease in nitrogenase activity with 0.005% yeast extract. In all the isolates, nitrogenase activity decreased with further increase of the yeast extract to 0.05%. The highest nitrogenase activity was observed in isolates C2HL2 and C7HL1, which had 16S rRNA gene sequences that were closely related to Novosphingobium sediminicola and Ochrobactrum intermedium, respectively.
机译：从工业品种（U-Thong 3； UT3），野生和咀嚼的甘蔗植物的叶子，茎和根中分离出内生固氮细菌，这些植物在无氮（N）的沙子中生长了6周。在无氮琼脂上获得了八十九种内生细菌的分离株。乙炔还原试验（ARA）检测了所有89种分离株中的固氮酶活性。表征了来自咀嚼甘蔗的三种分离株（C2HL2，C7HL1和C34MR1）和来自工业甘蔗（UT3R1）品种的一种分离株，并研究了它们对不同酵母提取物浓度的响应。观察到了三种不同的固氮酶活性响应。添加0.005％酵母提取物时，分离株C2HL2和C7HL1表现出主要的增加，C34MR1没有反应，而使用0.005％酵母提取物时UT3R1表现出明显的固氮酶活性下降。在所有分离物中，随着酵母提取物进一步增加至0.05％，固氮酶活性降低。在分离株C2HL2和C7HL1中观察到最高的固氮酶活性，分离株C2HL2和C7HL1的16S rRNA基因序列分别与间夜蛾新孢子虫和中间O虫密切相关。

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