• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Zhejiang University. Science. B >Application of derivative ratio spectrophotometry for determination of β-carotene and astaxanthin from Phaffia rhodozyma extract
【2h】

Application of derivative ratio spectrophotometry for determination of β-carotene and astaxanthin from Phaffia rhodozyma extract

机译：导数比分光光度法在红发夫提取物中β-胡萝卜素和虾青素的测定中的应用

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A derivative ratio spectrophotometric method was used for the simultaneous determination of β-carotene and astaxanthin produced from Phaffia rhodozyma. Absorbencies of a series of the standard carotenoids in the range of 441 nm to 490 nm demonstrated that their absorptive spectra accorded with Beer’s law and that the additivity when the concentrations of β-carotene and astaxanthin and their mixture were within the range of 0 to 5 µg/ml, 0 to 6 µg/ml, and 0 to 6 µg/ml, respectively. When the wavelength interval (Δλ) at 2 nm was selected to calculate the first derivative ratio spectra values, the first derivative amplitudes at 461 nm and 466 nm were suitable for quantitatively determining β-carotene and astaxanthin, respectively. Effect of divisor on derivative ratio spectra could be neglected; any concentration used as divisor in range of 1.0 to 4.0 µg/ml is ideal for calculating the derivative ratio spectra values of the two carotenoids. Calibration graphs were established for β-carotene within 0–6.0 µg/ml and for astaxanthin within 0–5.0 µg/ml with their corresponding regressive equations in: y=−0.0082x−0.0002 and y=0.0146x−0.0006, respectively. R-square values in excess of 0.999 indicated the good linearity of the calibration graphs. Sample recovery rates were found satisfactory (>99%) with relative standard deviations (RSD) of less than 5%. This method was successfully applied to simultaneous determination of β-carotene and astaxanthin in the laboratory-prepared mixtures and the extract from the Phaffia rhodozyma culture.
机译：用导数比分光光度法同时测定红发夫夫酵母产生的β-胡萝卜素和虾青素。一系列标准类胡萝卜素在441 nm至490 nm范围内的吸光度表明，它们的吸收光谱符合比尔定律，并且当β-胡萝卜素和虾青素及其混合物的浓度在0至5范围内时具有可加性微克/毫升，0至6微克/毫升和0至6微克/毫升。当选择2 nm处的波长间隔（Δλ）来计算一阶导数比光谱值时，在461 nm和466 nm处的一阶导数幅度分别适用于定量测定β-胡萝卜素和虾青素。除数对导数比谱的影响可以忽略不计；在1.0至4.0 µg / ml的范围内用作除数的任何浓度都是计算两个类胡萝卜素的导数比光谱值的理想选择。建立了β-胡萝卜素在0–6.0 µg / ml范围内和虾青素在0–5.0 µg / ml范围内的校准图，其相应的回归方程分别为：y = -0.0082x-0.0002和y = 0.0146x-0.0006。 R平方值超过0.999表示校准图具有良好的线性。发现样品回收率令人满意（> 99％），相对标准偏差（RSD）小于5％。该方法已成功地用于同时测定实验室制备的混合物和红发夫夫酵母培养物中的β-胡萝卜素和虾青素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号