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首页> 美国卫生研究院文献>Veterinary Medicine International >Ovine and Caprine Brucellosis (Brucella melitensis) in Aborted Animals in Jordanian Sheep and Goat Flocks
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Ovine and Caprine Brucellosis (Brucella melitensis) in Aborted Animals in Jordanian Sheep and Goat Flocks

机译：约旦绵羊和山羊群堕胎动物的绵羊和山羊布鲁氏菌病（Brucella melitensis）

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Two hundred and fifty five biological samples were collected from 188 animals (81 sheep and 107 goats) during the lambing season from September 2009 to April 2010 from the Mafraq region of Jordan. Sampled animals belonged to 93 sheep and goat flocks that had abortion cases in the region. One hundred and seven (41.9%) biological samples were positive for the omp2 primers that were able to identify all Brucella species in the collected samples which were obtained from 86 aborted animals (86/188 = 45.7%). Using the B. melitensis insertion sequence 711 (IS711) primers on the 107 omp2 positive samples, only 61 confirmed to be positive for B. melitensis. These positive samples were obtained from 28 sheep and 33 goats. The prevalence rate of B. melitensis was 27.1% (51/188) among aborted animals. For differentiation between vaccine strain and field strain infection, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using PstI endonuclease enzyme was used. Vaccination with Rev-1 in the last year (OR = 2.92, CI: 1.1–7.7) and grazing at common pasture (OR = 2.78, CI: 1.05–7.36) were statistically significant (P ≤ .05) risk factors positively associated with the occurrence of brucellosis in sheep and goat flocks.
机译：2009年9月至2010年4月的产羔季节，从约旦的马夫拉克地区采集了188只动物（81只绵羊和107只山羊）的255个生物样品。采样的动物属于该地区有流产病例的93只绵羊和山羊群。一百零七（41.9％）个生物学样品的omp2引物呈阳性，这些引物能够鉴定出从86只流产动物中采集的样品中的所有布鲁氏菌属（86/188 = 45.7％）。使用107 omp2阳性样品上的B. melitensis插入序列711（IS711）引物，只有61个被确认为B. melitensis阳性。这些阳性样品来自28只绵羊和33只山羊。在流产的动物中，melitensis的患病率为27.1％（51/188）。为了区分疫苗株和野毒株感染，使用了利用PstI核酸内切酶的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法。去年进行Rev-1疫苗接种（OR = 2.92，CI：1.1–7.7）和在普通牧场放牧（OR = 2.78，CI：1.05-7.36）在统计学上具有显着性（P≤.05）危险因素，与绵羊和山羊群中布鲁氏菌病的发生。

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