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首页> 美国卫生研究院文献>Viruses >Chronic Bee Paralysis Virus and Nosema ceranae Experimental Co-Infection of Winter Honey Bee Workers (Apis mellifera L.)
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Chronic Bee Paralysis Virus and Nosema ceranae Experimental Co-Infection of Winter Honey Bee Workers (Apis mellifera L.)

机译：慢性蜜蜂麻痹病毒和野菜Nosema ceranae实验性共感染冬季蜜蜂工（Apis mellifera L.）

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Chronic bee paralysis virus (CBPV) is an important viral disease of adult bees which induces significant losses in honey bee colonies. Despite comprehensive research, only limited data is available from experimental infection for this virus. In the present study winter worker bees were experimentally infected in three different experiments. Bees were first inoculated per os (p/o) or per cuticle (p/c) with CBPV field strain M92/2010 in order to evaluate the virus replication in individual bees. In addition, potential synergistic effects of co-infection with CBPV and Nosema ceranae (N. ceranae) on bees were investigated. In total 558 individual bees were inoculated in small cages and data were analyzed using quantitative real time RT-PCR (RT-qPCR). Our results revealed successful replication of CBPV after p/o inoculation, while it was less effective when bees were inoculated p/c. Dead bees harbored about 1,000 times higher copy numbers of the virus than live bees. Co-infection of workers with CBPV and N. ceranae using either method of virus inoculation (p/c or p/o) showed increased replication ability for CBPV. In the third experiment the effect of inoculation on bee mortality was evaluated. The highest level of bee mortality was observed in a group of bees inoculated with CBPV p/o, followed by a group of workers simultaneously inoculated with CBPV and N. ceranae p/o, followed by the group inoculated with CBPV p/c and the group with only N. ceranae p/o. The experimental infection with CBPV showed important differences after p/o or p/c inoculation in winter bees, while simultaneous infection with CBPV and N. ceranae suggesting a synergistic effect after inoculation.
机译：慢性蜜蜂麻痹病毒（CBPV）是成年蜜蜂的重要病毒性疾病，会引起蜜蜂群体的大量损失。尽管进行了广泛的研究，但从实验感染中只能获得有限的数据。在本研究中，冬季工蜂在三个不同的实验中被感染。首先用CBPV田间菌株M92 / 2010对每个（os / p）或每个表皮（p / c）接种蜜蜂，以评估病毒在单个蜜蜂中的复制。此外，还研究了与CBPV和Nosema ceranae（N. ceranae）共感染对蜜蜂的潜在协同作用。将总共​​558只蜜蜂接种在小笼子中，并使用实时定量RT-PCR（RT-qPCR）分析数据。我们的研究结果表明，P / O接种后CBPV成功复制，而P / c接种蜜蜂则效果较差。死蜂所藏病毒的拷贝数是活蜂的1000倍。使用任何一种病毒接种方法（p / c或p / o）将CBPV和cerenae猪共同感染工人，表明CBPV的复制能力增强。在第三个实验中，评估了接种对蜜蜂死亡率的影响。在一组接种CBPV p / o的蜜蜂中，蜜蜂死亡率最高，其次是一组同时接种CBPV和cerenae p.o的工人，其次是接种CBPV p / c和c.p.o的蜜蜂。仅含猪笼草的一组。 CBPV的实验性感染在冬季蜜蜂p / o或p / c接种后显示出重要差异，而同时感染CBPV和cer.ceranae则表明接种后具有协同效应。

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