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Identification of a Novel Gammaherpesvirus in Canada lynx (Lynx canadensis)

机译:在加拿大天猫座(加拿大天猫座)的一种新型伽玛疱疹病毒的鉴定。

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摘要

Gammaherpesviruses (GHVs) infect many animal species and are associated with lymphoproliferative disorders in some. Previously, we identified several novel GHVs in North American felids; however, a GHV had never been identified in Canada lynx (Lynx canadensis). We, therefore, hypothesized the existence of an unidentified GHV in lynx. Using degenerate nested and subsequently virus-specific PCR, we amplified and sequenced 3.4 kb of DNA from a novel GHV in lynx, which we named Lynx canadensis gammaherpesvirus 1 (LcaGHV1). Phylogenetic analysis determined that LcaGHV1 is a distinct GHV species belonging to the genus Percavirus. We then estimated the prevalence of LcaGHV1 in lynx by developing a PCR-based assay and detected LcaGHV1 DNA in 36% (95% CI: 22–53%) of lynx spleen DNA samples from Maine, USA and 17% (95% CI: 8–31%) from Newfoundland, Canada. The LcaGHV1 DNA sequences from Maine and Newfoundland lynx were nearly identical to each other (two nucleotide substitutions in 3.4 kb), suggesting that the unique lynx subspecies present on the island of Newfoundland (Lynx canadensis subsolanus) is infected with virus that very closely resembles virus found in mainland lynx. The potential ecologic and pathologic consequences of this novel virus for Canada lynx populations warrant further study.
机译:伽玛疱疹病毒(GHV)感染许多动物物种,并与某些动物的淋巴增生性疾病有关。之前,我们在北美猫科动物中发现了几种新颖的GHV。但是,加拿大天猫座(Lynx canadensis)从未发现GHV。因此,我们假设山猫中存在一个未知的GHV。使用简并的​​嵌套式和随后的病毒特异性PCR,我们从天猫座的一种新型GHV中扩增了3.4 kb的DNA,并对其进行了测序,我们将其命名为Lynx canadensis伽马疱疹病毒1(LcaGHV1)。系统发育分析确定LcaGHV1是属于Percavirus属的一个独特的GHV物种。然后,我们通过开发基于PCR的检测方法来估计LcaGHV1在山猫中的患病率,并在来自美国缅因州的36%(95%CI:22–53%)的山猫脾DNA样本中检测到LcaGHV1 DNA,并在17%(95%CI: 8–31%)来自加拿大纽芬兰。来自缅因州和纽芬兰天猫座的LcaGHV1 DNA序列彼此几乎相同(在3.4 kb中有两个核苷酸取代),这表明存在于纽芬兰岛(Lynx canadensis subsolanus)上的唯一天猫座亚种感染了非常类似于病毒的病毒在大陆中发现。这种新型病毒对加拿大山猫种群的潜在生态和病理后果值得进一步研究。

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