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首页> 美国卫生研究院文献>World Journal of Gastroenterology >Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma: An experimental study
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Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma: An experimental study

机译：经动脉化学栓塞对Walker-256移植性肝癌大鼠血管新生及血管内皮生长因子和碱性成纤维细胞生长因子表达的影响：实验研究

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AIM: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive responses. For example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted hepatoma.METHODS: Male Wistar rats were inoculated with Walker-256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-FU, 5-FU and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor VIII, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density (MVD) was counted in endothelial cells with positive factor VIII. Their expression levels were analyzed in conjunction with the pathologic features.RESULTS: While a smaller tumor volume was found in TACE group (F = 37.818, P < 0.001), no statistical differences between MVD and expression of VEGF and b-FGF were found among the 3 groups. MVD of the control group, chemotherapy group and chemoemoblization group was 80.84 ± 24.24, 83.05 ± 20.29 and 85.20 ± 23.91 (F = 0.193, P = 0.873), respectively. The positive expression of VEGF and b-FGF was 75%, 75%, 85% (χ² = 0.449, P = 0.799) and 30%, 25%, 30% (χ² = 0.141, P = 0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r = 0.552, P < 0.001).CONCLUSION: There has been little influence of lipiodol chemoembolization on the formation of tumor angiogenesis, but the development of neovascularization and expression of VEGF play important roles in establishment of collateral circulation and reconstruction of blood supply of residual cancer tissue.
机译：目的：经动脉化疗栓塞（TACE）后，残留的癌细胞处于广泛的缺氧甚至缺氧环境中。缺氧可导致适应性反应。例如，血管生成将帮助这些细胞存活。在这项研究中，我们研究了TACE对血管生成以及血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（b-FGF）表达的影响，并评估了它们与Walker-256移植肝癌的相关性。在肝左叶接种了Walker-256肿瘤。移植后第14天进行血管造影和经动脉化学栓塞术。将60只携带Walker-256肝癌的大鼠随机分为对照组，动脉输液组和TACE组。每组由二十只大鼠组成。分别通过肝动脉注入生理盐水，5-FU，5-FU和碘油。输注两周后，在常规石蜡包埋切片中通过免疫组织化学方法对因子VIII，VEGF和b-FGF进行染色。在具有阳性因子VIII的内皮细胞中计数微血管密度（MVD）。结果：TACE组肿瘤体积较小（F = 37.818，P <0.001），但MVD与VEGF和b-FGF的表达无统计学差异。 3组。对照组，化疗组和化学动员组的MVD分别为80.84±24.24、83.05±20.29和85.20±23.91（F = 0.193，P = 0.873）。 VEGF和b-FGF的阳性表达分别为75％，75％，85％（χ2 ＝ 0.449，P ＝ 0.799）和30％，25％，30％（χ2 ＝ 0.141，P ＝ 0.922）。统计分析表明，VEGF的表达与MVD呈正相关（r = 0.552，P <0.001）。结论：碘油化学栓塞对肿瘤血管生成的影响很小，但新血管形成的发展和VEGF的表达在建立侧支循环和重建残余癌组织的血液供应中起重要作用。

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