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首页> 美国卫生研究院文献>World Journal of Gastroenterology >Nuclear factor κB activity in patients with acute severe cholangitis
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Nuclear factor κB activity in patients with acute severe cholangitis

机译：急性重症胆管炎患者的核因子κB活性

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AIM: To determine the NF-κB activity in peripheral blood mononuclear cells (PBMC) in patients with acute cholangitis of severe type (ACST) and correlate the degree of NF-κB activation with severity of biliary tract infection and clinical outcome.METHODS: Twenty patients with ACST were divided into survivor group (13 cases) and nonsurvivor group (7 cases). Other ten patients undergoing elective gastrectomy or inguinal hernia repair were selected as control group. Peripheral blood samples were taken 24 h postoperatively. PBMC were separated by density gradient centrifugation, then nuclear proteins were isolated from PBMC, and Electrophoretic Mobility Shift Assay (EMSA) used determined. The results were quantified by scanning densitometer of a Bio-Image Analysis System and expressed as relative optical density (ROD). The levels of TNF-α, IL-6, and IL-10 in the plasma of patients with ACST and healthy control subjects were determined by using an enzyme-linked immunoassay (ELISA).RESULTS: The NF-κB activity was 5.02 ± 1.03 in nonsurvivor group, 2.98 ± 0.51 in survivor group and 1.06 ± 0.34 in control group. There were statistical differences in three groups (P < 0.05). The levels of TNF-α and IL-6 in plasma were (498 ± 53) ng·L-1 and (587 ± 64) ng·L-1 in nonsurvivor group, (284 ± 32) ng·L-1 and (318 ± 49) ng·L-1 in survivor group and (89 ± 11) ng·L-1 and (102 ± 13) ng·L-1 in control group. All patients with ACST had increased levels of TNF-α and IL-6, which were manyfold greater than those of control group, and there was an evidence of significantly higher levels in those of nonsurvivor group than that in survivor group (P < 0.05). The levels of IL-10 in plasma were (378 ± 32) ng·L-1, (384 ± 37) ng·L-1 and (68 ± 11) ng·L-1 in three groups, respectively. All patients had also increased levels of IL-10 when compared with control group (P < 0.05), but the IL-10 levels were not significantly higher in nonsurvivors than in survivors (P > 0.05).CONCLUSION: NF-κB activity in PBMC in patients with ACST increases markedly and the degree of NF-κB activation is correlated with severity of biliary tract infection and clinical outcome.
机译：目的：确定重症急性胆管炎（ACST）患者外周血单个核细胞（PBMC）的NF-κB活性，并将NF-κB的活化程度与胆道感染的严重程度和临床结局相关联。方法：二十ACST患者分为生存组（13例）和非生存组（7例）。选择其他十​​例行选择性胃切除术或腹股沟疝修补术的患者作为对照组。术后24小时采集外周血。通过密度梯度离心分离PBMC，然后从PBMC中分离核蛋白，并确定使用的电泳迁移率测定法（EMSA）。通过生物图像分析系统的扫描密度计对结果进行定量，并表示为相对光密度（ROD）。采用酶联免疫吸附试验（ELISA）测定ACST患者和健康对照者血浆中TNF-α，IL-6和IL-10的水平。结果：NF-κB活性为5.02±1.03非存活者组为2.98±0.51，对照组为1.06±0.34。三组之间有统计学差异（P <0.05）。非存活者血浆中TNF-α和IL-6水平为（498±53）ng·L -1 和（587±64）ng·L -1 幸存者组为（284±32）ng·L -1 和（318±49）ng·L -1 ，（89±11）ng·L <对照组为sup> -1 和（102±13）ng·L -1 。所有ACST患者的TNF-α和IL-6水平均升高，比对照组高出许多倍，并且有证据表明非存活组的TNF-α和IL-6水平显着高于存活组（P <0.05） 。血浆中IL-10的水平为（378±32）ng·L -1 ，（384±37）ng·L -1 和（68±11） ng·L -1 分为三组。与对照组相比，所有患者的IL-10水平也均升高（P <0.05），但非存活者的IL-10水平并未显着高于存活者（P> 0.05）。结论：PBMC中NF-κB活性ACST患者的血浆中NF-κB活化程度与胆道感染的严重程度和临床结局相关。

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