AIM: To investigate the inhibitory effect of a Chinese herb medicine Astragali radix (AR) on growth of different cancer cell lines.METHODS: To observe the in vitro effects of AR on tumor cell proliferation by trypan blue exclusion, MTS method and tritium thymidine incorporation assay. Apoptosis was detected by DNA ladder method.RESULTS: The inhibition rates of AR on the cell respiration of AGS, KATOIII, HT29, MDA231, MEL7 and MEL14 were 68.25%, 62.36%, 22.8%, 27.69%, 2.85% and 5.14% respectively at the concentration of 100 ug/mL; it inhibited AGS DNA synthesis by 87.33% at the concentration of 50 ug/mL. The inhibitory effect on AGS was time-and dose-dependent. AR did not induce apoptosis in AGS cells.CONCLUSION: AR specifically inhibits gastric cancer cells growth in vitro and the mechanism is mainly cytostatic but not cytotoxic or inducing apoptosis.
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机译：目的：探讨中药黄芪对不同癌细胞系生长的抑制作用。方法：通过台盼蓝排斥法，MTS法和掺入胸腺嘧啶tri观察AR对肿瘤细胞增殖的体外作用。分析。结果：AR对AGS，KATOIII，HT29，MDA231，MEL7和MEL14细胞呼吸的抑制率分别为68.25％，62.36％，22.8％，27.69％，2.85％和5.14％。浓度为100 ug / mL;在50 ug / mL的浓度下，它抑制了AGS DNA的合成达87.33％。对AGS的抑制作用是时间和剂量依赖性的。结论：AR在体外能特异性抑制胃癌细胞的生长，其机制主要是抑制细胞生长，而不是抑制细胞毒性或诱导细胞凋亡。
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