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Effects of astragali radix on the growth of different cancer cell lines

机译:黄芪对不同癌细胞系生长的影响

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AIM: To investigate the inhibitory effect of a Chinese herb medicine Astragali radix (AR) on growth of different cancer cell lines. METHODS: To observe the in vitro effects of AR on tumor cell proliferation by trypan blue exclusion, MTS method and tritium thymidine incorporation assay. Apoptosis was detected by DNA ladder method. RESULTS: The inhibition rates of AR on the cell respiration of AGS, KATOIII, HT29, MDA231, MEL7 and MEL14 were 68.25 %, 62.36 %, 22.8 %, 27.69 %, 2.85 % and 5.14 % respectively at the concentration of 100 ug/ml; it inhibited AGS DNA synthesis by 87.33 % at the concentration of 50 ug/ml. The inhibitory effect on AGS was time-and dose-dependent. AR did not induce apoptosis in AGS cells. CONCLUSION: AR specifically inhibits gastric cancer cells growth in vitro and the mechanism is mainly cytostatic but not cytotoxic or inducing apoptosis.
机译:目的:探讨中药黄芪对不同癌细胞系生长的抑制作用。方法:采用台盼蓝排斥法,MTS法和胸苷tri掺入法观察AR对肿瘤细胞增殖的体外作用。用DNA阶梯法检测细胞凋亡。结果:在100 ug / ml浓度下,AR对AGS,KATOIII,HT29,MDA231,MEL7和MEL14的细胞呼吸的抑制率分别为68.25%,62.36%,22.8%,27.69%,2.85%和5.14%。 ;在50 ug / ml的浓度下,它抑制了AGS DNA的合成达87.33%。对AGS的抑制作用是时间和剂量依赖性的。 AR没有诱导AGS细胞凋亡。结论:AR在体外能特异性抑制胃癌细胞的生长,其机制主要是抑制细胞生长,而不是抑制细胞毒性或诱导细胞凋亡。

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