AIM: To investigate the effects of traditional Chinese medicinal enema (TCME) on inflammatory and immune response of colonic mucosa of rats with ulcerative colitis (UC), and to observe the pathogenic mechanism.METHODS: Thirty UC rats, induced by intestinal enema together with 2.4-dinitrochlorobenzene (DNCB) and acetic acid, were randomly divided into 3 groups, i.e., G I, G II and G III. Groups G I and G II were administered with TCME and salazosulfapyridine enema (SASPE), respectively. Group G III was clystered with only normal saline (NSE), served as control. Group G IV was taken from normal rats as reference, once daily, from the 7th day after the establishment of UC for total 28 d. Interleukin-6 (IL-6) in the colonic mucosa was assayed by 3H-TdR incorporation assay. Colonic mucosal lymphocyte subpopulation adhesive molecules, CD4+CD11a+, CD4+CD18+, CD8+CD11a+, CD8+CD18+ (LSAM), tumor necrosis factor (TNF)-α, and interferon-γ (IFN-γ), were detected by enzyme linked immunosorbent assay (ELISA). Moreover, the expression of TNF-α mRNA and IFN-γ mRNA in colonic mucosa were detected by polymerase chain reaction (RT-PCR).RESULTS: Before therapies, in model groups, G I, G II and G III, levels of IL-6, TNF-α, IFN-γ, CD8+CD11a+ and CD8+CD18+ were significantly different (38.29 ± 2.61 U/mL, 16.54 ± 1.23 ng/L, 8.61 ± 0.89 ng/L, 13.51% ± 2.31% and 12.22% ± 1.13%, respectively) compared to those in G IV group (31.56 ± 2.47 U/mL, 12.81 ± 1.38 ng/L, 5.28 ± 0.56 ng/L, 16.68% ± 1.41% and 16.79% ± 1.11%, respectively). After therapeutic enemas, in G I group, the contents of IL-6 (32.48 ± 2.53 U/m), TNF-α (13.42 ± 1.57 ng/L) and IFN-γ (5.87 ± 0.84 ng/L) were reduced; then, the contents of CD8+CD11a+ (16.01% ± 1.05 %) and CD8+CD18+ (16.28% ± 0.19%) were raised. There was no significant difference between groups G I and G IV, but the difference between groups G I and G II was quite obvious (P < 0.05). The expressions of TNF-α mRNA and IFN-γ mRNA in group G III were much higher than those of group G IV, but those in group G I were significantly suppressed by TCME therapy.CONCLUSION: Ulcerative colitis is related to colonic regional mucosal inflammatory factors and immune imbalance. TCME can effectively inhibit regional mucosal inflammatory factors and improve their disorder of immunity.
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机译：目的：探讨中药灌肠（TCME）对溃疡性结肠炎（UC）大鼠结肠黏膜炎性和免疫反应的影响，并探讨其致病机理。方法：30只UC灌肠联合肠灌肠诱导大鼠2.4-二硝基氯苯（DNCB）和乙酸随机分为3组，即GI，G II和G III。 G I组和G II组分别给予TCME和Salazosulfapyridine灌肠剂（SASPE）。 G III组仅用生理盐水（NSE）灌胃，作为对照。从建立UC后第7天开始，每天一次，从正常大鼠中取出G IV组作为参考，共28天。用 3 H-TdR掺入法检测结肠黏膜中的白介素-6（IL-6）。结肠粘膜淋巴细胞亚群粘附分子CD4 + CD11a + ，CD4 + CD18 + ，CD8 + CD11a + ，CD8 + CD18 + （LSAM），肿瘤坏死因子（TNF）-α和干扰素-γ（通过酶联免疫吸附测定（ELISA）检测IFN-γ）。此外，通过聚合酶链反应（RT-PCR）检测结肠黏膜中TNF-α和IFN-γ的表达。结果：治疗前，模型组GI，G II和G III的IL-水平6，TNF-α，IFN-γ，CD8 + CD11a + 和CD8 + CD18 + 有显着差异与G IV组（31.56±2.47 U / mL）相比（分别为38.29±2.61 U / mL，16.54±1.23 ng / L，8.61±0.89 ng / L，13.51％±2.31％和12.22％±1.13％） ，分别为12.81±1.38 ng / L，5.28±0.56 ng / L，16.68％±1.41％和16.79％±1.11％）。灌肠后，GI组中IL-6（32.48±2.53 U / m），TNF-α（13.42±1.57 ng / L）和IFN-γ（5.87±0.84 ng / L）的含量降低；然后，CD8 + CD11a + （16.01％±1.05％）和CD8 + CD18 + 的内容（提高了16.28％±0.19％）。 G I和G IV组之间没有显着差异，但G I和G II组之间的差异非常明显（P <0.05）。结论：溃疡性结肠炎与结肠局部粘膜炎性因子有关，溃疡性结肠炎与GIII组相比，GIII组的TNF-α和IFN-γmRNA表达明显高于GIV组，但GI组明显降低。和免疫失衡。 TCME可以有效抑制局部粘膜炎性因子并改善其免疫功能。
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