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首页> 美国卫生研究院文献>World Journal of Gastroenterology >Plasma free amino acid profiling of esophageal cancer using high-performance liquid chromatography spectroscopy
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Plasma free amino acid profiling of esophageal cancer using high-performance liquid chromatography spectroscopy

机译：高效液相色谱法测定食管癌的血浆游离氨基酸谱

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AIM: To perform plasma free amino acid (PFAA) profiling of esophageal squamous cell carcinoma (ESCC) patients at different pathological stages and healthy subjects.METHODS: Plasma samples from ESCC patients (n = 51) and healthy control adults (n = 60) were analyzed by high-performance liquid chromatography (HPLC). The ESCC patients included moderate/poorly-differentiation (n = 24), lymph node metastasis (n = 17) and clinical stage > Ib2 (n = 36). Partial least squares discriminant analysis was performed to demonstrate that the PFAA metabolic patterns enabled discrimination between ESCC patients and controls, and the Student t test was applied to assess significant differences in PFAA concentrations between the two groups.RESULTS: There were significant differences in the PFAA profiles between controls and ESCC patients. Compared with healthy controls, the levels of Asp, Glu, Gly, His, Thr, Tau, Ala, Met, Ile, Leu, and Phe were decreased in ESCC patients, but Cys was increased. There exists a strong correlation between PFAA profiles and clinicopathological characteristics in ESCC patients. The levels of many PFAAs (i.e., Glu, Asp, Ser, Gly, Tau, Ala, Tyr, Val, Ile, and Leu) were related to pathological grading, lymph node metastasis, and ESCC clinical stage. Very good discrimination between ESCC patients and control subjects was achieved by multivariate modeling of plasma profiles.CONCLUSION: HPLC-based plasma profiling analysis was shown to be an effective approach to differentiate between ESCC patients and controls. PFAA profiles may have potential value for screening or diagnosing ESCC.
机译：目的：对处于不同病理阶段和健康受试者的食管鳞状细胞癌（ESCC）患者进行血浆游离氨基酸（PFAA）分析。方法：ESCC患者（n = 51）和健康对照成年人（n = 60）的血浆样本用高效液相色谱（HPLC）分析。 ESCC患者包括中度/差分化（n = 24），淋巴结转移（n = 17）和临床阶段> Ib2（n = 36）。进行偏最小二乘判别分析以证明PFAA的代谢模式能够区分ESCC患者和对照组，并应用Student t检验评估两组之间PFAA浓度的显着差异。结果：PFAA存在显着差异对照和ESCC患者之间的概况。与健康对照组相比，ESCC患者的Asp，Glu，Gly，His，Thr，Tau，Ala，Met，Ile，Leu和Phe的水平降低，但Cys升高。食管鳞癌患者的PFAA谱与临床病理特征之间存在很强的相关性。许多PFAAs（即Glu，Asp，Ser，Gly，Tau，Ala，Tyr，Val，Ile和Leu）的水平与病理分级，淋巴结转移和ESCC临床阶段有关。通过对血浆分布图进行多变量建模，可以很好地区分ESCC患者和对照对象。结论：基于HPLC的血浆谱分析是区分ESCC患者和对照的有效方法。 PFAA配置文件可能具有筛选或诊断ESCC的潜在价值。

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