首页>
外国专利>
Human plasma free amino acids profile using pre-column derivatizing reagent- 1-naphthylisocyanate and high performance liquid chromatographic method
Human plasma free amino acids profile using pre-column derivatizing reagent- 1-naphthylisocyanate and high performance liquid chromatographic method
展开▼
机译：柱前衍生试剂-1-萘基异氰酸酯与高效液相色谱法测定人血浆游离氨基酸
展开▼
页面导航
摘要
著录项
相似文献
摘要
After many decades, 1-Naphthylisocyanate (NIC) has been identified as the most ideal pre column derivatization fluorescent tag for reversed phase Liquid Chromatographic (HPLC) analysis of all free amino acids (AA) in biological samples. NIC forms very stable derivatives with all AAs in one minute. Using NIC, the first, most simple, robust, sensitive (femto mole), and economical high pressure binary gradient, HPLC method, has been developed. It estimates 35 (and 2 internal standards) AAs in human plasma in record shortest time of 20 minutes and has been validated for precision (n=16, 6%), accuracy 95 %, linearity (0 to 1200 μM/L), and analyzing normal and abnormal patients. It can provide with in 20 minutes the first and best plasma free AAs profile that includes Homocysteine, Cysteine, Alloisoleucine, and Cystathionine and a 27 AAs profile using a blood spot (3 μl plasma). A sample can be analyzed with in one hour of its arrival in the laboratory.
展开▼
