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Phenolic contents and antioxidant activity of ethanolic extract of Capparis spinosa

机译:刺五加乙醇提取物的酚含量和抗氧化活性

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摘要

Caper plant (Capparis spinosa) extracts have been associated with diverse biological activities including anti-oxidant properties. In this work, we characterized the hydro-ethanolic extract obtained from C. spinosa leaves [hydroethanolic extract of C. spinosa (HECS)] by analyzing the content in anti-oxidant compounds such as polyphenols, flavonoids and anthocyanins. Further, we evaluated HECS antioxidant activities in vitro using bleaching of 1,1-diphenyl-2-picrylhydrazyl radical and ABTS test as well as by pretreatment of HeLa cells exposed to Fe2+ or H2O2. Our findings indicate that HECS contains high amount of total phenolic compounds and high levels of flavonoids and anthocyanins. Furthermore, HECS exhibited antioxidant activity in both chemical and biological tests. Specially, pretreatment of HeLa cells with different concentrations of the extract conferred protection against lipid peroxidation and modulated activities of two antioxidant enzymes, SOD and catalase. These results revealed HECS antioxidant effects and suggest that C. spinosa leaves are a potential source of natural antioxidant molecules with possible applications in industry and medicine.
机译:雀跃植物(Capparis spinosa)提取物已与多种生物活性相关,包括抗氧化特性。在这项工作中,我们通过分析多酚,类黄酮和花色苷等抗氧化剂的含量,对从棘孢叶得到的水乙醇提取物[棘孢藻的乙醇提取物(HECS)]进行了表征。此外,我们使用1,1-二苯基-2-吡啶并肼基自由基的漂白和ABTS测试,以及通过预处理暴露于Fe 2 + 或H2O2的HeLa细胞,评估了HECS的体外抗氧化活性。我们的发现表明,HECS含有大量的总酚类化合物以及大量的类黄酮和花色苷。此外,HECS在化学和生物学测试中均表现出抗氧化活性。特别地,用不同浓度的提取物对HeLa细胞进行预处理可防止脂质过氧化并调节两种抗氧化酶SOD和过氧化氢酶的活性。这些结果揭示了HECS的抗氧化作用,并表明刺藻叶是天然抗氧化剂分子的潜在来源,可能在工业和医学中得到应用。

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