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首页> 美国卫生研究院文献>Antioxidants >A Comparative Study on Phenolic Content Antioxidant Activity and Anti-Inflammatory Capacity of Aqueous and Ethanolic Extracts of Sorghum in Lipopolysaccharide-Induced RAW 264.7 Macrophages
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A Comparative Study on Phenolic Content Antioxidant Activity and Anti-Inflammatory Capacity of Aqueous and Ethanolic Extracts of Sorghum in Lipopolysaccharide-Induced RAW 264.7 Macrophages

机译：脂多糖诱导的原料264.7巨噬细胞高粱水性和乙醇萃取物酚类含量抗氧化活性和抗炎能力的比较研究

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Sorghum is an important cereal with diverse phenolic compounds that have potential health promoting benefits. The current study comparatively characterized the phenolic contents of two novel black-seeded sorghum lines (SC84 and PI570481) using different extraction systems (water, ethanol and their acidified counterparts) and evaluated their antioxidant and anti-inflammatory activities. Phenolic compositions were determined by spectrophotometric assays and HPLC analysis. Antioxidant activities were assessed by radical scavenging effects on nitric oxide (NO) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, and the oxygen radical absorbance capacity (ORAC). Anti-inflammatory capacity was estimated by measuring levels of pro-inflammatory markers produced by lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Results showed that effects of solvent types and HCl on extraction efficiency differed among phenolic compounds and sorghum samples. Tannins were the most dominant polyphenols in the studied extracts (11.11–136.11 mg epicatechin equivalent/g sorghum). Sorghum extracts exerted more potent scavenging activity on DPPH than NO radicals. In LPS-activated RAW 264.7 cells, sorghum extracts dose-dependently inhibited the production of NO, interleukin-6 (IL-6), and intracellular reactive oxygen species (ROS), with ethanolic extracts showing greater anti-inflammatory activity. Positive correlations were noted between tannin content and DPPH radical scavenging activity, and anti-inflammatory capacity. These results suggest the potential role of tannin-rich sorghum extracts against inflammation and associated diseases.
机译：高粱是一种重要的谷物，具有各种酚类化合物，具有潜在的健康促进益处。目前的研究表征了使用不同的提取系统（水，乙醇及其酸化对应物）的两种新型黑色种子线（SC84和PI570481）的酚醛含量，并评估其抗氧化剂和抗炎活性。通过分光光度法测定和HPLC分析测定酚醛组合物。通过对一氧化氮（NO）和2,2-二苯基-1-富铬（DPPH）自由基的自由基清除效应来评估抗氧化活性，以及​​氧自由基吸收能力（ORAC）。通过测量通过脂多糖（LPS）产生的促炎症标志物的水平估计抗炎能力 - 诱导的原料264.7巨噬细胞。结果表明，酚类化合物和高粱样品的提取效率对溶剂类型和HCl对萃取效率的影响。单宁是研究提取物中最占优势的多酚（11.11-136.11 mg EpicateChin当量/ g高粱）。高粱提取物在DPPH中施加了更有效的清除活性而不是无基类。在LPS-活化的原料264.7细胞中，高粱提取物剂量依赖性抑制NO，白细胞介素-6（IL-6）和细胞内反应性氧物质（ROS）的产生，乙醇提取物显示出更大的抗炎活性。在单宁含量和DPPH激进的活性和抗炎能力之间指出了正相关性。这些结果表明富含丹宁高粱提取物对炎症和相关疾病的潜在作用。

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