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首页> 美国卫生研究院文献>Contrast Media Molecular Imaging >Carboxyl of Poly(DL-lactide-co-glycolide) Nanoparticles of Perfluorooctyl Bromide for Ultrasonic Imaging of Tumor
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Carboxyl of Poly(DL-lactide-co-glycolide) Nanoparticles of Perfluorooctyl Bromide for Ultrasonic Imaging of Tumor

机译：全氟辛基溴化物的聚（DL-丙交酯-共-乙交酯）纳米粒子的羧基用于肿瘤超声成像

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摘要

Perfluorooctyl bromide (PFOB) enclosed nanoparticles (NPs) as ultrasonic contrasts have shown promising results in the recent years. However, NPs display poor contrast enhancement in vivo. In this work, we used the copolymers poly(lactide-co-glycolide) carboxylic acid (PLGA-COOH) and poly(lactide-co- glycolide) poly(ethylene glycol) carboxylic acid (PLGA-PEG-COOH) as a shell to encapsulate PFOB to prepare a nanoultrasonic contrast agent. The NPs were small and uniform (210.6 ± 2.9 nm with a polydispersity index of 0.129 ± 0.016) with a complete shell nuclear structure under the transmission electron microscopy (TEM). In vitro, when concentration of NPs was ≥10 mg/ml and clinical diagnostic frequency was ≥9 MHz, NPs produced intensive enhancement of ultrasonic gray-scale signals. NPs could produce stable and obvious gray enhancement with high mechanical index (MI) (MI > 0.6). In vivo, the NPs offered good ultrasound enhancement in tumor after more than 24 h and optical imaging also indicated that NPs were mainly located at tumor site. Subsequent analysis confirmed that large accumulation of fluorescence was observed in the frozen section of the tumor tissue. All these results caused the conclusion that NPs encapsulated PFOB has achieved tumor-selective imaging in vivo.
机译：近年来，全氟辛基溴（PFOB）包裹的纳米颗粒（NPs）作为超声造影剂已显示出令人鼓舞的结果。但是，NPs在体内显示差的对比度增强。在这项工作中，我们使用共聚物聚（丙交酯-乙交酯）羧酸（PLGA-COOH）和聚（丙交酯-乙交酯）聚（乙二醇）羧酸（PLGA-PEG-COOH）作为壳封装PFOB以制备纳米超声造影剂。在透射电子显微镜（TEM）下，NP小且均匀（210.6±2.9 nm，多分散指数为0.129±0.016），具有完整的壳核结构。在体外，当NP的浓度≥10μg/ ml且临床诊断频率≥9μMHz时，NP会产生超声灰度信号的强烈增强。 NP可以在高机械指数（MI）（MI> 0.6）下产生稳定且明显的灰度增强。在体内，NPs在超过24h的时间后在肿瘤中提供了良好的超声增强，光学成像也表明NPs主要位于肿瘤部位。随后的分析证实，在肿瘤组织的冷冻切片中观察到大量的荧光积累。所有这些结果得出结论：NPs封装的PFOB已在体内实现了肿瘤选择性成像。

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