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首页> 美国卫生研究院文献>Clinical and Experimental Immunology >The potentiation and inhition by autologous red cells and platelets of human lymphocyte transformation induced by pokeweed mitogen concanavalin A mercuric chloride antigen and mixed leucocyte culture
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The potentiation and inhition by autologous red cells and platelets of human lymphocyte transformation induced by pokeweed mitogen concanavalin A mercuric chloride antigen and mixed leucocyte culture

机译：商陆有丝分裂素刀豆伴刀豆球蛋白A氯化汞抗原和混合白细胞培养诱导的自体红细胞和血小板对人淋巴细胞转化的增强和抑制作用

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Prior work on the potentiation of phytohaemagglutinin-induced human lymphocyte transformation by autologous red blood cells (RBC) and platelets prompted the present report.Studies were performed on the effects of autologous RBC and platelets upon lymphocyte transformation induced by a variety of mitogenic stimuli, utilizing human peripheral blood lymphocytes free of RBC and platelets as the starting cell preparation. The mitogenic activity of pokeweed mitogen (PWM) is potentiated to maximum of 300% by RBC: lymphocyte ratios of 0·5–20:1. Potentiation of lymphocyte transformation induced by concanavalin A (CON-A) does not occur, but >50% inhibition is found at RBC: lymphocyte ratios of 5–10:1. The mitogenic activities of both PWM and CON-A are potentiated 200–400% by the presence of platelets in a platelet: lymphocyte ratio of 3–10:1; higher doses of platelets are consistently (PWM) or occasionally (CON-A) less stimulatory and may actually cause inhibition of lymphocyte transformation. In contrast to these phytomitogens, lymphocyte transformation induced by antigen (PPD) in optimum mitogenic doses is not potentiated by either RBC or platelets. RBC do not alter HgCl2-induced lymphocyte transformation, but platelets cause modest potentiation. In mixed leucocyte cultures the presence of either autologous or allogeneic RBC or platelets markedly inhibits transformation (platelets > RBC).These observations demonstrate that the presence of mitotically inert particles such as autologous RBC or platelets may profoundly affect lymphocyte transformation induced by a variety of mitogenic stimuli. Possible explanations for the mechanism of these effects are discussed. Since many methods of lymphocyte collection and preparation yield populations of lymphocytes significantly contaminated with RBC and/or platelets, comparative studies of human lymphocyte transformation in health and disease which do not remove these cellular contaminants or control their number are subject to the most tentative interpretation.
机译：自发性红细胞（RBC）和血小板对植物血凝素诱导的人淋巴细胞转化的增强作用已有研究进展。本研究利用多种有丝分裂原刺激物对自体红细胞和血小板对淋巴细胞转化的影响进行了研究。不含红细胞和血小板的人外周血淋巴细胞作为起始细胞制剂。 RBC：淋巴细胞比率为0·5–20：1，商陆有丝分裂原（PWM）的有丝分裂活性可增强至最大300％。伴刀豆球蛋白A（CON-A）诱导的淋巴细胞转化没有增强作用，但是在RBC：淋巴细胞比率为5-10：1时发现抑制> 50％。血小板与淋巴细胞的比例为3-10：1时，存在血小板时，PWM和CON-A的促有丝分裂活性增强200-400％。较高剂量的血小板持续（PWM）或偶尔（CON-A）刺激较少，实际上可能导致淋巴细胞转化受到抑制。与这些植物有丝分裂原相反，在最佳有丝分裂剂量下，由抗原（PPD）诱导的淋巴细胞转化不会被RBC或血小板增强。 RBC不会改变HgCl2诱导的淋巴细胞转化，但血小板会引起适度的增强作用。在混合白细胞培养物中，自体或同种异体RBC或血小板的存在均显着抑制转化（血小板> RBC）。这些观察结果表明，有丝分裂惰性颗粒（如自体RBC或血小板）的存在可能会深刻影响多种有丝分裂原诱导的淋巴细胞转化刺激。讨论了这些作用机理的可能解释。由于许多收集和制备淋巴细胞的方法会产生被RBC和/或血小板严重污染的淋巴细胞群，因此对人类淋巴细胞在健康和疾病中转化的比较研究并未去除这些细胞污染物或控制其数量，这受到了最初步的解释。

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