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首页> 美国卫生研究院文献>Clinical and Experimental Immunology >Increased interferon-gamma (IFN-γ) IL-10 and decreased IL-4 mRNA expression in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE)
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Increased interferon-gamma (IFN-γ) IL-10 and decreased IL-4 mRNA expression in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE)

机译：系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中干扰素-γ（IFN-γ）IL-10的表达升高和IL-4 mRNA表达的降低

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摘要

Cytokines are important regulators of lymphocyte function in SLE. However, the profile of Th1 and Th2 cytokines produced by circulating lymphocytes in human SLE has not been clearly elucidated. The aim of the present study was to characterize the gene expressions of the Th1-type cytokine IFN-γ, and the Th2-type cytokines IL-10 and IL-4 in PBMC of 15 patients with SLE and 10 healthy individuals by a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). Our results showed that expression of IFN-γ (P = 0·0004) and IL-10 (P = 0·002) transcripts were significantly increased in PBMC of patients with SLE compared with healthy controls. By contrast, expression of IL-4 transcripts in PBMC of patients with SLE was significantly decreased compared with the healthy controls (P = 0·0008). Primary sources of IL-10 were B cells and monocytes, with variable contribution of T cells as detected in various fractions of PBMC of patients with SLE (P = 0·049). These findings support the hypothesis that the enhanced production of IFN-γ by mononuclear cells may trigger inflammatory responses, together with the enhanced production of IL-10 resulting in autoantibody production by B cells in human SLE.
机译：细胞因子是SLE中淋巴细胞功能的重要调节剂。但是，尚未清楚阐明人SLE中循环淋巴细胞产生的Th1和Th2细胞因子的概况。本研究的目的是通过半定量逆转分析15例SLE患者和10例健康个体的PBMC中Th1型细胞因子IFN-γ和Th2型细胞因子IL-10和IL-4的基因表达。转录酶聚合酶链反应（RT-PCR）。我们的结果表明，与健康对照组相比，SLE患者的PBMC中IFN-γ（P = 0·0004）和IL-10（P = 0·002）的表达显着增加。相比之下，与健康对照组相比，SLE患者PBMC中IL-4转录物的表达显着降低（P = 0·0008）。 IL-10的主要来源是B细胞和单核细胞，在SLE患者的PBMC的各个部分中检测到T细胞的贡献均不同（P = 0·049）。这些发现支持以下假说：单核细胞增加的IFN-γ产生可能触发炎症反应，以及IL-10产生的增加，导致人SLE中B细胞产生自身抗体。

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