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首页> 美国卫生研究院文献>Cell Stress Chaperones >Cloning of the heat shock protein 90 and 70 genes from the beet armyworm Spodoptera exigua and expression characteristics in relation to thermal stress and development
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Cloning of the heat shock protein 90 and 70 genes from the beet armyworm Spodoptera exigua and expression characteristics in relation to thermal stress and development

机译：甜菜夜蛾甜菜夜蛾热休克蛋白90和70基因的克隆及其与热应激和发育的关系

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Two full-length cDNAs of heat shock protein (HSP) genes (Se-hsp90 and Se-hsp70) were cloned from the beet armyworm, Spodoptera exigua, and their expression was investigated in relation to cold shock, heat shock, and development. The open reading frames of Se-hsp90 and Se-hsp70 are 2,154 and 2,004 bp in length, encoding polypeptides of 717 and 667 amino acids with a molecular mass of 82.6 and 72.5 kDa, respectively. Both genes showed high similarity to their counterparts in other species. Transcriptional expression profiles revealed that both genes were significantly up-regulated under thermal stress. However, the temperature at which expression level became significantly higher than that of controls varied between genes. Intensity of response to temperature was more intense for Se-hsp70 than for Se-hsp90, regardless of temperature or developmental stage. However, intensities of response to temperature of either Se-hsp90 or Se-hsp70 varied with developmental stage. The basal expression of both genes was highest in young larvae and decreased with age. Translational expression of Se-Hsp70 was observed by using Western blot, the expression profiles of Se-Hsp70 protein were in high agreement with those of Se-hsp70 RNA under heat or cold stress in larvae and pupae. However, it does not completely accord with that of Se-hsp70 RNA expression during development without thermal stress. These results indicated that, in addition to heat shock responses, both Se-hsp90 and Se-hsp70 might be involved in development.
机译：从甜菜夜蛾Spodoptera exigua克隆了两个热休克蛋白（HSP）基因的全长cDNA（Se-hsp90和Se-hsp70），并研究了它们与冷休克，热休克和发育的关系。 Se-hsp90和Se-hsp70的开放阅读框长度为2,154和2,004 bp，分别编码717和667个氨基酸的多肽，分子量分别为82.6和72.5 kDa。两种基因都显示出与其他物种的高度相似性。转录表达谱显示，两个基因在热胁迫下均显着上调。但是，基因之间表达水平显着高于对照的温度不同。无论温度或发育阶段如何，Se-hsp70的温度响应强度都比Se-hsp90的强度高。然而，Se-hsp90或Se-hsp70对温度的响应强度随发育阶段而变化。这两个基因的基础表达在幼虫中最高，并随年龄下降。通过Western blot观察Se-Hsp70蛋白的翻译表达，结果表明，在热和冷胁迫下，幼虫和h中Se-Hsp70蛋白的表达谱与Se-hsp70 RNA的表达谱高度吻合。但是，它与没有热应激的Se-hsp70 RNA表达并不完全一致。这些结果表明，除了热休克反应，Se-hsp90和 Se-hsp70 都可能参与了发育。

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