• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Cell Stress Chaperones >Characterization functional analysis and expression levels of three carbonic anhydrases in response to pH and saline–alkaline stresses in the ridgetail white prawn Exopalaemon carinicauda
【2h】

Characterization functional analysis and expression levels of three carbonic anhydrases in response to pH and saline–alkaline stresses in the ridgetail white prawn Exopalaemon carinicauda

机译：pH尾对虾白–Exopalaemon carinicauda中三种碳酸酐酶响应pH和盐碱胁迫的表征功能分析和表达水平

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Carbonate alkalinity, salinity, and pH are three important stress factors for aquatic animals in saline–alkaline water. Carbonic anhydrases (CAs) catalyze the reversible reaction of CO2 reported to play an important role in the acid–base regulation in vertebrates. To explore the molecular mechanism of CAs efficacy in shrimp after their transfer into saline–alkaline water, the cDNAs of three CAs (EcCAc, EcCAg, and EcCAb) were cloned from Exopalaemon carinicauda. Sequence analysis showed that EcCAc and EcCAg both possessed a conserved α-CA domain and a proton acceptor site, and EcCAb contained a Pro-CA domain. Tissue expression analysis demonstrated that EcCAc and EcCAg were most abundantly in gills, and EcCAb was highly expressed in muscle. The cumulative mortalities remained below 25% under exposure to pH (pH 6 and pH 9), low salinity (5 ppt), or high carbonate alkalinity (5 and 10 mmol/L) after 72 h of exposure. However, mortalities increased up to 70% under extreme saline–alkaline stress (salinity 5 ppt, carbonate alkalinity 10 mmol/L, and pH 9) after 14 days of exposure. The EcCAc and EcCAg expressions in gills were significantly upregulated during the early period of pH and saline–alkaline stresses, while the EcCAb expressions showed no regular or large changes. The two-way ANOVA found significant interactions between salinity and carbonate alkalinity observed in EcCAc, EcCAg, and EcCAb expressions (p < 0.05). Furthermore, an RNA interference experiment resulted in increased mortality of EcCAc- and EcCAg-silenced prawns under saline–alkaline stress. EcCAc knockdown reduced expressions of Na+/H+ exchanger (EcNHE) and sodium bicarbonate cotransporter (EcNBC), and EcCAg knockdown reduced EcCAc, EcNHE, EcNBC, and V-type H+-ATPase (EcVTP) expressions. These results suggest EcCAc and EcCAg as important modulators in response to pH and saline–alkaline stresses in E. carinicauda.Electronic supplementary materialThe online version of this article (10.1007/s12192-019-00987-z) contains supplementary material, which is available to authorized users.
机译：碳酸盐碱度，盐度和pH是盐水-碱性水中水生动物的三个重要应激因素。碳酸酐酶（CAs）催化据报道在脊椎动物的酸碱调节中起重要作用的CO2可逆反应。为了研究CAs转移到盐碱水中后在虾中的功效机理，从Exopalaemon carinicauda克隆了三个CA（EcCAc，EcCAg和EcCAb）的cDNA。序列分析表明，EcCAc和EcCAg均具有保守的α-CA结构域和质子受体位点，而EcCAb具有Pro-CA结构域。组织表达分析表明，EcCAc和EcCAg在g中含量最高，而EcCAb在肌肉中高表达。在暴露72小时后，暴露于pH（pH 6和pH 9），低盐度（5ppt）或高碳酸盐碱度（5和10 mmol / L）下，累积死亡率仍低于25％。然而，暴露14天后，在极端的盐碱压力下（盐度5ppt，碳酸盐碱度10mmol / L和pH 9），死亡率增加了70％。在pH和盐碱胁迫的早期，g中EcCAc和EcCAg的表达明显上调，而EcCAb的表达则没有规则或较大的变化。双向方差分析发现EcCAc，EcCAg和EcCAb表达中盐度和碳酸盐碱度之间存在显着相互作用（ p <0.05）。此外，RNA干扰实验导致 EcCAc -和 EcCAg -沉默的虾在盐碱胁迫下的死亡率增加。 EcCAc 组合物可降低Na + / H + 交换子（ EcNHE ）和碳酸氢钠共转运蛋白（ EcNBC ）和 EcCAg 组合可降低 EcCAc ， EcNHE ， EcNBC 和V型H + -ATPase（ EcVTP ）表达式。这些结果表明， EcCAc 和 EcCAg 是响应 E的pH和盐碱胁迫的重要调节剂。电子补充材料本文的在线版本（10.1007 / s12192-019-00987-z）包含补充材料，授权用户可以使用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号