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首页> 外文期刊>Fish & Shellfish Immunology >The roles of Na+/K+-ATPase alpha-subunit gene from the ridgetail white prawn Exopalaemon carinicauda in response to salinity stresses
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The roles of Na+/K+-ATPase alpha-subunit gene from the ridgetail white prawn Exopalaemon carinicauda in response to salinity stresses

机译：脊尾白虾Exopalaemon carinicauda Na + / K + -ATPaseα-亚基基因对盐分胁迫的响应

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摘要

Na+/K+-LATPase (NAK) is one important transporter protein and plays a key role in maintaining osmotic homeostasis in low and high salinity acclimation in variety of crustacean species. The ridgetail white prawn Exopalaemon carinicauda is an euryhaline and economic shrimp species in China, but it remains unclear about its mechanism of salinity adaption. In this study, a full-length of Na+/K+-ATPase alpha-subunit (alpha-NAK) cDNA was cloned from E. carinicauda by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of alpha-NAK was of 3680 bp, containing an open reading frame (ORF) of 3030 bp encoding a polypeptide of 1009 amino acids with the predicted molecular weight of 112.27 kDa. Eight transmembrane domains and two sites of phosphorylation and ATP binding were identified in E. carinicauda alpha-NAK. BLAST analysis revealed that the sequence of alpha-NAK amino acids of E. carinicauda shared more than 75% homologies with those of other crustacean. Real time quantitative RT-PCR analysis indicated that E. carinicauda alpha-NAK gene could be detected in all the tested tissues with highest expression level in gill. The expression profiles of E. carinicauda alpha-NAK transcripts were analyzed in gill and hepatopancreas tissues after salinity stresses. The results showed that the expression level of E. carinicauda alpha-NAK gene in both gill and hepatopancreas reached peak at different time after low and high salinity stresses, and showed different expression profiles. The expression profiles of proPO transcripts in gills after salinity stresses also indicated alpha-NAK and proPO played synergistic actions for salinity responses in E. carinicauda. These results indicated that E. carinicauda alpha-NAK involved in stress responses against salinity. (C) 2014 Elsevier Ltd. All rights reserved.
机译：Na + / K + -LATPase（NAK）是一种重要的转运蛋白，在各种甲壳类动物的低盐度和高盐度适应中，在维持渗透稳态方面起着关键作用。脊尾白虾Exopalaemon carinicauda是中国的一种淡水虾和经济虾，但尚不清楚其盐分适应机制。在这项研究中，通过使用cDNA末端的快速扩增（RACE）方法，从大肠杆菌中克隆了全长Na + / K + -ATPaseα-亚基（α-NAK）cDNA。 alpha-NAK的全长cDNA为3680 bp，包含3030 bp的开放阅读框（ORF），编码1009个氨基酸的多肽，预测分子量为112.27 kDa。在carinicauda alpha-NAK中鉴定出八个跨膜结构域和两个磷酸化和ATP结合位点。 BLAST分析显示，大肠杆菌的α-NAK氨基酸序列与其他甲壳类动物具有超过75％的同源性。实时定量RT-PCR分析表明，在all中表达水平最高的所有被测组织中均可检测到大肠杆菌。盐分胁迫后，在car和肝胰腺组织中分析了大肠杆菌的α-NAK转录本的表达谱。结果表明，在低盐和高盐度胁迫下，car和肝胰腺中E. carinicauda alpha-NAK基因的表达水平在不同时间达到峰值，并表现出不同的表达特征。盐度胁迫后g中proPO转录本的表达谱也表明α-NAK和proPO在大肠杆菌中的盐度响应中发挥了协同作用。这些结果表明大肠杆菌Carinicauda alpha-NAK参与了针对盐度的胁迫反应。 （C）2014 Elsevier Ltd.保留所有权利。

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