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Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering

机译:无需两个抗体模块即可加载两个模块实现抗体缀合物的受控组装

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摘要

The generation of antibody conjugates with a loading of two modules is desirable for a host of reasons. Whilst certain antibody engineering approaches have been useful in the preparation of such constructs, a reliable method based on a native antibody scaffold without the use of enzymes or harsh oxidative conditions has hitherto not been achieved. The use of native antibodies has several advantages in terms of cost, practicality, accessibility, time and overall efficiency. Herein we present a novel, reliable method of furnishing antibody conjugates with a loading of two modules starting from a native antibody scaffold.
机译:由于许多原因,需要产生带有两个模块的抗体偶联物。尽管某些抗体工程方法已可用于制备此类构建体,但迄今为止尚未实现基于天然抗体支架而不使用酶或苛刻氧化条件的可靠方法。就成本,实用性,可及性,时间和整体效率而言,使用天然抗体具有多个优点。本文中,我们提出了一种新颖,可靠的方法,以天然抗体支架为起点,通过两个模块的负载提供抗体偶联物。

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