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Cell-Seeded Collagen Matrix–Supported Autologous Chondrocyte Transplantation (ACT-CS)

机译:细胞接种的胶原蛋白基质支持的自体软骨细胞移植(ACT-CS)

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摘要

Objective:Autologous chondrocyte transplantation has become an established therapy for full-thickness cartilage defects. Cell-seeded collagen matrix–supported autologous chondrocyte transplantation (ACT-CS) has been introduced as a modification of conventional ACT, which allows easier handling and is intended to combine the advantages of using a cell suspension (i.e., cell viability and mitotic activity) with the stability and self-containment provided by a matrix of biomaterials. Unlike other techniques and products, this seeding step can be easily applied using a porcine collagen type I/III membrane and autologous chondrocytes in an operating room setting. Although some suturing is required, this technique provides the distinct advantage of not requiring a water-tight seal of the bilayer membrane, as is required using the classic cell suspension technique. Comparable to other modifications of ACT, the ACT-CS procedure requires a specific surgical technique that focuses on the following important details: (1) accurate debridement of the cartilage defect; (2) preparation of the cells, and seeding and containment of the cells within the transplantation site; and (3) sealing and suturing around the defect.
机译:目的:自体软骨细胞移植已成为治疗全层软骨缺损的公认方法。作为传统ACT的改良,引入了由细胞播种的胶原蛋白基质支持的自体软骨细胞移植(ACT-CS),该方法使操作更容易,并且旨在结合使用细胞悬液的优势(例如,细胞活力和有丝分裂活性)以及生物材料基质提供的稳定性和自我控制。与其他技术和产品不同,此接种步骤可在手术室环境中使用猪I / III型胶原蛋白膜和自体软骨细胞轻松进行。尽管需要进行一些缝合,但是该技术具有不像传统细胞悬浮技术那样需要水密密封双层膜的独特优势。与ACT的其他修改相比,ACT-CS程序需要一种专门的手术技术,其重点是以下重要细节:(1)软骨缺损的准确清创术; (2)准备细胞,并在移植部位内接种和容纳细胞; (3)对缺损处进行缝合缝合。

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