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首页> 美国卫生研究院文献>Cancers >PC 12 Pheochromocytoma Cell Response to Super High Frequency Terahertz Radiation from Synchrotron Source
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PC 12 Pheochromocytoma Cell Response to Super High Frequency Terahertz Radiation from Synchrotron Source

机译：PC 12嗜铬细胞瘤细胞对同步辐射源超高频太赫兹辐射的反应

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High frequency (HF) electromagnetic fields (EMFs) have been widely used in many wireless communication devices, yet within the terahertz (THz) range, their effects on biological systems are poorly understood. In this study, electromagnetic radiation in the range of 0.3–19.5 × 1012 Hz, generated using a synchrotron light source, was used to investigate the response of PC 12 neuron-like pheochromocytoma cells to THz irradiation. The PC 12 cells remained viable and physiologically healthy, as confirmed by a panel of biological assays; however, exposure to THz radiation for 10 min at 25.2 ± 0.4 °C was sufficient to induce a temporary increase in their cell membrane permeability. High-resolution transmission electron microscopy (TEM) confirmed cell membrane permeabilization via visualisation of the translocation of silica nanospheres (d = 23.5 ± 0.2 nm) and their clusters (d = 63 nm) into the PC 12 cells. Analysis of scanning electron microscopy (SEM) micrographs revealed the formation of atypically large (up to 1 µm) blebs on the surface of PC 12 cells when exposed to THz radiation. Long-term analysis showed no substantial differences in metabolic activity between the PC 12 cells exposed to THz radiation and untreated cells; however, a higher population of the THz-treated PC 12 cells responded to the nerve growth factor (NGF) by extending longer neurites (up to 0–20 µm) compared to the untreated PC12 cells (up to 20 µm). These findings present implications for the development of nanoparticle-mediated drug delivery and gene therapy strategies since THz irradiation can promote nanoparticle uptake by cells without causing apoptosis, necrosis or physiological damage, as well as provide a deeper fundamental insight into the biological effects of environmental exposure of cells to electromagnetic radiation of super high frequencies.
机译：高频（HF）电磁场（EMF）已在许多无线通信设备中广泛使用，但是在太赫兹（THz）范围内，人们对它们对生物系统的影响知之甚少。在这项研究中，使用同步加速器光源产生的0.3–19.5×10 12 Hz范围内的电磁辐射用于研究PC 12神经元样嗜铬细胞瘤细胞对THz辐射的响应。一组生物学分析证实，PC 12细胞保持活力和生理健康。然而，在25.2±0.4°C的THz辐射下暴露10分钟足以诱导它们的细胞膜通透性暂时增加。高分辨率透射电子显微镜（TEM）通过可视化二氧化硅纳米球（d = 23.5±0.2 nm）及其簇（d = 63 nm）进入PC 12细胞的转移，证实了细胞膜的通透性。扫描电子显微镜（SEM）显微照片的分析显示，当暴露于THz辐射时，PC 12细胞表面上会形成非典型的大气泡（最大1 µm）。长期分析显示，暴露于THz辐射的PC 12细胞与未经处理的细胞之间在代谢活性方面没有实质性差异；然而，与未经处理的PC12细胞（至多20 µm）相比，经过THz处理的PC 12细胞具有更大的种群对神经生长因子（NGF）的反应，其延伸的神经突更长（至0–20 µm）。这些发现对纳米介导的药物传递和基因治疗策略的发展具有暗示意义，因为太赫兹辐射可以促进细胞吸收纳米颗粒而不会引起凋亡，坏死或生理损伤，并且可以提供对环境暴露的生物学效应的更深层次的基本见解。细胞受到超高频电磁辐射的影响。

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