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首页> 美国卫生研究院文献>The Journal of Physiology >The Tetrodotoxin-Resistant Na+ Channel Nav1.8 is Essential for the Expression of Spontaneous Activity in Damaged Sensory Axons of Mice
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The Tetrodotoxin-Resistant Na+ Channel Nav1.8 is Essential for the Expression of Spontaneous Activity in Damaged Sensory Axons of Mice

机译：抗河豚毒素的Na +通道Nav1.8对于受损小鼠感觉轴突中自发表达的活性至关重要。

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The tetrodotoxin-resistant sodium channel α subunit, Nav1.8, is exclusively expressed in primary sensory neurons and is suggested to play a role in the generation of ectopic action potentials after axonal injury and thereby contribute to neuropathic pain. Here we investigated the involvement of Nav1.8 in ectopic impulse generation in damaged axons by examining spontaneous activity and mechanosensitivity in neuromas formed by section of the saphenous nerve in Nav1.8 null mice and in their wild-type littermates. We recorded 522 identified units from 24 neuromas in vitro at two time points, 8–11 days (median 10 days) and 19–29 days (median 22 days) post-operatively. At ≈10 days, neither genotype showed spontaneous activity, but a significantly higher proportion of fibres were mechanosensitive in wild-type (54 %) compared to Nav1.8 null neuromas (18 %). At ≈22 days, 19 % of fibres recorded in wild-type neuromas showed spontaneous activity, whereas only one fibre of the 238 (0.4 %) recorded in neuromas taken from null mice showed ongoing activity. In recordings at ≈22 days, a similar proportion of fibres were mechanosensitive in wild-type and Nav1.8 null neuromas (51 and 46 %, respectively). We conclude that Nav1.8 is essential for the expression of spontaneous activity in damaged sensory axons, and may also contribute to the development of ectopic mechanosensitivity.
机译：抗河豚毒素的钠通道α亚基Nav1.8仅在原代感觉神经元中表达，并提示其在轴突损伤后在异位动作电位的产生中起作用，从而导致神经性疼痛。在这里，我们通过检查Nav1.8无效小鼠及其野生型同窝仔的隐神经部分形成的神经瘤的自发活动和机械敏感性，研究了Nav1.8在受损轴突异位冲动产生中的参与。我们在两个时间点，即术后8-11天（中位数10天）和19-29天（中位数22天）在两个时间点记录了来自24个神经瘤的522个已识别单位。在≈10天时，两种基因型均未显示出自发活动，但与Nav1.8无神经瘤（18％）相比，野生型（54％）对机械敏感的纤维比例高得多。在≈22天时，野生型神经瘤中记录的19％的纤维表现出自发活动，而从无效小鼠中采集的神经瘤中记录的238种纤维中只有一根（0.4％）表现出持续活动。在大约22天的记录中，在野生型和Nav1.8无效神经瘤中，类似比例的纤维对机械敏感（分别为51％和46％）。我们得出结论，Nav1.8对于受损感觉轴突中自发活动的表达必不可少，并且也可能有助于异位机械敏感性的发展。

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