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Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature

机译：通过phi29 DNA聚合酶和新型引物在高温下特异性和完整地扩增人类基因组并提高产量

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BackgroundsWhole genome amplification (WGA) is a practical solution to eliminate molecular analysis limitations associated with genomic DNA (gDNA) quantity. Different methods have been developed to amplify the whole genome, including primer extension preamplification (PEP), degenerate oligonucleotide primed PCR (DOP-PCR), and multiple displacement amplification (MDA). Each of these methods has its own merits and limitations.

机译：背景全基因组扩增（WGA）是消除与基因组DNA（gDNA）数量相关的分子分析限制的实用解决方案。已经开发出不同的方法来扩增整个基因组，包括引物延伸预扩增（PEP），简并寡核苷酸引发的PCR（DOP-PCR）和多置换扩增（MDA）。这些方法中的每一种都有其优点和局限性。

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期刊名称 BMC Research Notes
作者
Osama Alsmadi; Fadi Alkayal; Dorota Monies; Brian F Meyer;
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年(卷),期 2009(2),-1
年度 2009
页码 48
总页数 7
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专利

1. DNA polymerase template switching at specific sites on the phi29 genome causes the in vivo accumulation of subgenomic phi29 DNA molecules. [J] . Murthy V, Meijer WJ, Blanco L, Molecular Microbiology . 1998,第3期

机译：在phi29基因组上特定位置的DNA聚合酶模板转换导致体内亚基因组phi29 DNA分子的积累。
2. Amplification of human DNA by primers targeted to leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction. [J] . Vergel C, Walker J, Saravia NG The American Journal of Tropical Medicine and Hygiene . 2005,第4期

机译：通过靶向利什曼原虫运动塑料DNA的引物扩增人类DNA，以及通过聚合酶链反应检测寄生虫时的基因组后考虑。
3. Improved KIR gene and HLA-C KIR ligand sequence-specific primer polymerase chain reaction genotyping using whole genome amplification. [J] . Chainonthee W, Bottcher G, Gagne K, Tissue antigens. . 2010,第2期

机译：使用全基因组扩增改进的KIR基因和HLA-C KIR配体序列特异性引物聚合酶链反应基因分型。
4. IMPROVEMENT OF LIGATION-MEDIATED POLYMERASE CHAIN REACTION (LM-PCR) METHOD FOR GENOME WALKING WITH A SINGLE GENE-SPECIFIC PRIMER [C] . Keina Kozono, Ayako Aoki, Masayoshi Tsukahara, Annual Meeting of the Japanese Association for Animal Cell Technology . 2006

机译：用单个基因特异性引物进行结扎介导的聚合酶链反应（LM-PCR）方法的结扎介导的聚合酶链反应（LM-PCR）方法
5. Repetitive DNA sequence elements and DNA polymerases modulate instability in the human genome. [D] . Walsh, Erin. 2014

机译：重复的DNA序列元件和DNA聚合酶调节人类基因组中的不稳定性。
6. Dual functional Phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA [O] . Xiao-Yu Li, Yi-Chen Du, Yu-Peng Zhang, -1

机译：双功能Phi29 DNA聚合酶触发的指数滚环扩增技术用于序列特异性检测长链基因组DNA中嵌入的目标DNA
7. Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature [O] . Monies Dorota, Alkayal Fadi, Alsmadi Osama, 2009

机译：通过phi29 DNA聚合酶和新型引物在高温下特异性和完整地扩增人类基因组，并提高产量
8. Specificity of Polymerase Chain Amplification Reactions for Human Immunodeficiency Virus Type 1 DNA Sequences. (Reannouncement with New Availability Information). [R] . Bell, J., Ratner, L. 1989

机译：人类免疫缺陷病毒1型DNa序列的聚合酶链扩增反应的特异性。（重新公布新的可用性信息）。

Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature

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