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Specificity of Polymerase Chain Amplification Reactions for Human Immunodeficiency Virus Type 1 DNA Sequences. (Reannouncement with New Availability Information).

机译：人类免疫缺陷病毒1型DNa序列的聚合酶链扩增反应的特异性。（重新公布新的可用性信息）。

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The polymerase chain amplification reaction (PCR) is a sensitive, specific, and quantitative assay of human immunodeficiency virus type 1 (HIV-1). The assay was performed with polymerases from Escherichia coli or Thermus aquaticus (Taq). A single pair of oligonucleotide primers within the long terminal repeat (LTR) sequences were used to detect HIV-1 sequences in infected cell cultures and fresh tissues of the large majority of infected individuals. The amplified product was a faithful copy of the LTR sequence. Utilization of a subsaturating number of cycles of amplification allowed quantitation of HIV-1 DNA sequences. HIV-1 infection may cause no symptoms, an acute influenza-like illness, lymphadenopathy syndrome, acquired immunodeficiency disease syndrome (AIDS)-related complex (ARC), or AIDS. A high proportion of infected individuals progress from asymptomatic to symptomatic stages of disease. There are considerable data to suggest that virus load and immunosuppression are major determinants of disease stage.

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作者
Bell, J.; Ratner, L.;
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年度 1989
页码 1-9
总页数 9
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Amplification; Assaying; Cells(Biology); Chain reactions; Cultures(Biology); Escherichia coli; Immunosuppression; Inflammation; Lymph nodes; Signs and symptoms; Tissues(Biology); Viruses; Reprints; Aids; Polymerase chain reaction; Deoxyribonucleic acids; HIV-1; Thermus; HIV long terminal repeat;

机译：扩增;测定;细胞（生物学）;链反应;培养物（生物学）;大肠杆菌;免疫抑制;炎症;淋巴结;体征和症状;组织（生物学）;病毒;重印;辅助;聚合酶链反应;脱氧核糖核酸; HIV -1; Thermus; HIV长末端重复;

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专利

1. Failure of serial human immunodeficiency virus type 1 DNA polymerase chain reactions to identify human immunodeficiency virus type 1 clade A/G. [J] . Obaro SK, Losikoff P, Harwell J, The Pediatric infectious disease journal . 2005,第2期

机译：连续的1型人类免疫缺陷病毒DNA聚合酶链反应未能鉴定1型人类免疫缺陷病毒进化枝A / G。
2. Detection of polymerase chain reaction-amplified human immunodeficiency virus type 1 proviral DNA with a digoxigenin-labeled RNA probe and an enzyme-linked immunoassay. [J] . Y He, F Coutlée, P Saint-Antoine, Journal of Clinical Microbiology . 1993,第5期

机译：用洋地黄毒苷标记的RNA探针和酶联免疫法检测聚合酶链反应扩增的1型人类免疫缺陷病毒原病毒DNA。
3. Detection of human immunodeficiency virus type 1 DNA by polymerase chain reaction amplification and capture hybridization in microtiter wells. [J] . G H Keller, D P Huang, M M Manak Journal of Clinical Microbiology . 1991,第3期

机译：通过聚合酶链反应扩增检测人类免疫缺陷病毒1型DNA，并在微量滴定孔中捕获杂交。
4. DEVELOPMENT OF A POLYMERASE CHAIN REACTION-BASED ASSAY FOR THE DETECTION OF KOI HERPESVIRUS DNA IN FORMALIN FIXED, WAX EMBEDDED ARCHIVE TISSUES [C] . P.F. Dixon, C.L. Joiner, R.-M. Le Deuff, International Conference of the European Aquaculture Society . 2004

机译：基于聚合酶链反应的基于聚合酶链式的测定，用于检测福尔马林固定的KOI Herpesvirus DNA，蜡嵌入式档案组织
5. Development of polymerase chain reaction amplification assay as a screening test for identifying dairy herds infected with bovine viral diarrhea virus [D] . Radwan, Gamal Soliman 1994

机译：聚合酶链反应扩增分析法的开发，用于鉴定感染牛病毒性腹泻病毒的奶牛群
6. Detection of polymerase chain reaction-amplified human immunodeficiency virus type 1 proviral DNA with a digoxigenin-labeled RNA probe and an enzyme-linked immunoassay. [O] . Y He, F Coutlée, P Saint-Antoine, 1993

机译：用洋地黄毒苷标记的RNA探针和酶联免疫法检测聚合酶链反应扩增的1型人类免疫缺陷病毒原病毒DNA。
7. Detection of polymerase chain reaction-amplified human immunodeficiency virus type 1 proviral DNA with a digoxigenin-labeled RNA probe and an enzyme-linked immunoassay [O] . Y He, F Coutlée, P Saint-Antoine, 1993

机译：用Digoxigenin标记的RNA探针和酶联免疫测定的聚合酶链反应扩增的人免疫缺陷病毒型1型荧光DNA的检测
8. Cryptic Species in the Anopheles (Nyssorhynchus) albitarsis (Diptera: Culicidae) Complex: Incongruence Between Random Amplified Polymorphic DNA- Polymerase Chain Reaction Identification and Analysis of Mitochondrial DNA COI Gene Sequences. [R] . Lehr, M. A., Kilpatrick, C. W., Wilkerson, R. C., 2005

机译：按蚊（Nyssorhynchus）albitarsis（双翅目：蚊科）复合体中的隐蔽物种：随机扩增多态DNa-聚合酶链反应鉴定和线粒体DNa COI基因序列分析之间的不一致。

Specificity of Polymerase Chain Amplification Reactions for Human Immunodeficiency Virus Type 1 DNA Sequences. (Reannouncement with New Availability Information).

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