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首页> 美国卫生研究院文献>Proceedings of the Royal Society B: Biological Sciences >Recovery of mechano-electrical transduction in rat cochlear hair bundles after postnatal destruction of the stereociliar cross-links
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Recovery of mechano-electrical transduction in rat cochlear hair bundles after postnatal destruction of the stereociliar cross-links

机译：出生后的纤毛交联破坏后大鼠耳蜗发束的机电转导恢复

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摘要

Mechano-electrical transduction (MET) in the stereocilia of outer hair cells (OHCs) was studied in newborn Wistar rats using scanning electron microscopy to investigate the stereociliar cross-links, Nomarski laser differential interferometry to investigate stereociliar stiffness and by testing the functionality of the MET channels by recording the entry of fluorescent dye, FM1-43, into stereocilia. Preparations were taken from rats on their day of birth (P0) or 1–4 days later (P1–P4). Hair bundles developed from the base to the apex and from the inner to outer OHC rows. MET channel responses were detected in apical coil OHCs on P1. To study the possible recovery of MET after disrupting the cross-links, the same investigations were performed after the application of Ca2+ chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) and allowing the treated samples to recover in culture medium for 0–20 h. We found that the structure and function were abolished by BAPTA. In P0–P1 samples, structural recovery was complete and the open probability of MET channels reached control values. In P3–P4 samples, complete recovery only occurred in OHCs of the outermost row. Although our results demonstrate an enormous recovery potential of OHCs in the postnatal period, the structural component restricts the potential for therapy in patients.
机译：使用扫描电子显微镜研究新生Wistar大鼠外毛细胞的立体纤毛（OHCs）中的机电转导（MET），以研究立体纤毛的交联，Nomarski激光微分干涉术研究立体纤毛的刚度并通过测试其功能MET通道通过记录荧光染料FM1-43进入到纤毛中来。在大鼠出生当天（P0）或1–4天后（P1–P4）从大鼠中提取制剂。发束从基部到根尖以及从OHC内部到外部排发。在P1的顶端线圈OHC中检测到MET通道响应。为了研究破坏交联后MET的可能回收率，在应用Ca 2 + 螯合剂1,2-双（邻氨基苯氧基）乙烷-N，N之后，进行了相同的研究， N'，N'-四乙酸（BAPTA），使处理后的样品在培养基中恢复0-20小时。我们发现BAPTA取消了结构和功能。在P0–P1样品中，结构恢复已完成，MET通道的打开概率达到了控制值。在P3–P4样品中，完全恢复仅发生在最外排的OHC中。尽管我们的结果表明，产后OHC的恢复潜力巨大，但结构成分限制了患者治疗的潜力。

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