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Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection

机译:板栗枯萎病感染后美国板栗(Castanea dentata)和中国板栗(Castanea mollissima)的转录组比较

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摘要

Background1471-2229-9-51American chestnut (Castanea dentata) was devastated by an exotic pathogen in the beginning of the twentieth century. This chestnut blight is caused by Cryphonectria parasitica, a fungus that infects stem tissues and kills the trees by girdling them. Because of the great economic and ecological value of this species, significant efforts have been made over the century to combat this disease, but it wasn't until recently that a focused genomics approach was initiated. Prior to the Genomic Tool Development for the Fagaceae project, genomic resources available in public databases for this species were limited to a few hundred ESTs. To identify genes involved in resistance to C. parasitica, we have sequenced the transcriptome from fungal infected and healthy stem tissues collected from blight-sensitive American chestnut and blight-resistant Chinese chestnut (Castanea mollissima) trees using ultra high throughput pyrosequencing.
机译:背景技术1471-2229-9-51美国板栗(Castanea dentata)在20世纪初被外来病原体破坏。栗叶枯萎病是由寄生Cryphonectria parasitica引起的,这种真菌会感染茎组织,并束缚它们杀死树木。由于该物种具有巨大的经济和生态价值,因此在过去的一个世纪里人们为抗击这种疾病做出了巨大的努力,但是直到最近,才开始着眼于基因组学。在开展Fagaceae项目的基因组工具之前,公共数据库中针对该物种的可用基因组资源仅限于数百个EST。为了鉴定抗寄生虫梭状芽胞杆菌的基因,我们使用超高通量焦磷酸测序技术对从对白叶枯病敏感的美国板栗和对白叶枯病的中国板栗(Castanea mollissima)树收集的真菌感染的健康茎组织进行了转录组测序。

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