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Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality

机译:胚胎大西洋大比目鱼(Hippoglossus hippoglossus L.)转录组的分析揭示了母本转录本是胚胎质量的潜在标志

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摘要

BackgroundCommercial Atlantic halibut (Hippoglossus hippoglossus) farming is restricted by variable oocyte quality, slow growth, and early maturation of male fish. Maternally transferred components regulate early developmental processes; therefore, they have an effect on the future viability of the embryo. Using a newly developed Agilent 10 k custom-made oligonucleotide array, we profiled components of the transcriptome involved in immune defence as well as germline and muscle development during early developmental stages: 8-cell embryos (8CS), germ ring stage (GR), 10-somite stage (10SS), and hatched embryos (HT). In addition, we identified differentially expressed transcripts in low (≤9 ± 3% hatching) and high (≥86 ± 3°% hatching) quality eggs at 8CS to identify potential maternal markers for embryo quality.
机译:背景技术商业大西洋大比目鱼(Hippoglossus hippoglossus)的养殖受到卵母质量变化,生长缓慢和雄鱼早期成熟的限制。母体转移的成分调节早期发育过程;因此,它们对胚胎的未来生存能力有影响。使用新开发的安捷伦10 k定制寡核苷酸阵列,我们分析了在早期发育阶段涉及免疫防御以及种系和肌肉发育的转录组组件:8细胞胚胎(8CS),生殖环阶段(GR), 10次​​阶段(10SS)和孵出的胚胎(HT)。此外,我们在8CS鉴定出质量低(≤9%±3%孵化)和质量高(≥86%±3%孵化)的差异表达转录本,以鉴定潜在的母体胚胎品质标记。

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