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Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

机译：免疫球蛋白人源化大鼠的抗原特异性单B细胞分选和表达克隆：一种快速通用的产生高亲和力和区分性人单克隆抗体的方法

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BackgroundThere is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied a strategy for the isolation of antigen-specific B cells using tetramerized proteins and single-cell sorting followed by reconstruction of human mAbs by RT-PCR and expression cloning.

机译：背景技术对于生物医学应用，单克隆抗体（mAb）的需求不断增长，由于它们在患者中的免疫原性降低，因此特别需要完全人结合的抗体。我们已应用四聚体蛋白和单细胞分选方法分离抗原特异性B细胞的策略，然后通过RT-PCR和表达克隆重建人mAb。

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期刊名称 BMC Biotechnology
作者
Laure-Hélène Ouisse; Laetitia Gautreau-Rolland; Marie-Claire Devilder; Michael Osborn; Melinda Moyon; Jonathan Visentin; Frank Halary; Marianne Bruggemann; Roland Buelow; Ignacio Anegon; Xavier Saulquin;
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作者单位

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年(卷),期 2017(17),-1
年度 2017
页码 3
总页数 17
原文格式 PDF
正文语种
中图分类应用微生物学;
关键词
Humanized rats Human antibodies Tetramers pMHC Cytofluorimetry;

机译：人源化大鼠;人抗体;四聚体;pMHC;细胞荧光法;

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专利

1. Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies [J] . Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, BMC Biotechnology . 2017,第1期

机译：免疫球蛋白人源化大鼠的抗原特异性单B细胞分选和表达克隆：一种快速，通用的产生高亲和力和区分性人单克隆抗体的方法
2. Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. [J] . Weitkamp JH, Kallewaard N, Kusuhara K, Journal of Immunological Methods . 2003,第1a2期

机译：从用荧光病毒样颗粒选择的单个抗原特异性B细胞中产生轮状病毒重组人单克隆抗体。
3. Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting. [J] . Bohm E, Grillari J, Voglauer R, Journal of Immunological Methods . 2005,第1a2期

机译：建立通过流式细胞仪分选快速生成抗人蛋白质SNEV（hNMP200）单克隆抗体的策略。
4. Efficient Acquisition of Antigen-Specific Human Monoclonal Antibody by Using Peripheral Blood Mononuclear Cells Immunized In Vitro Efficient Cloning of Human Monoclonal Antibody Gene [C] . Shinei Matsumoto, Makiko Yamashita, Yoshinori Katakura, Annual Meeting of the Japanese Association for Animal Cell Technology . 2009

机译：通过使用在体外克隆人单克隆抗体基因的体外克隆的外周血单核细胞高效采集抗原特异性人单克隆抗体
5. Transcriptional regulation of the production of a humanized monoclonal antibody by CHO cells [D] . Jiang, Zhou 2007

机译：CHO细胞产生人源化单克隆抗体的转录调控
6. The rapid generation of recombinant functional monoclonal antibodies from individual antigen-specific bone marrow-derived plasma cells isolated using a novel fluorescence-based method [O] . Alison M Clargo, Ashley R Hudson, Welcome Ndlovu, 2014

机译：使用基于荧光的新方法从单个抗原特异性骨髓来源的浆细胞中快速生成重组功能性单克隆抗体
7. Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies [O] . Ouisse, Laure-Hélène, Gautreau-Rolland, Laetitia, Devilder, Marie-Claire, 2017

机译：免疫球蛋白人源化大鼠的抗原特异性单B细胞分选和表达克隆：一种快速，通用的产生高亲和力和区分性人单克隆抗体的方法

Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

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