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首页> 美国卫生研究院文献>Biotechnology Research International >Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth
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Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth

机译：曲霉产α-淀粉酶的发酵生产和热稳定性及其在棉布脱浆中的应用潜力。

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摘要

The production of extracellular amylase was investigated employing our laboratory isolate, Aspergillus niger sp. MK 07 and effect of process variables on enzyme production, was studied in a fermentor. It was found that amylase production was maximum when the fermentor volume was maintained at 70%, rate of agitation at 250 rpm, air supply at 2.5 vvm, inoculum concentration of 10%, and a pH of 5.0. Highest enzyme production obtained under all optimized conditions was 1734 U/mL with sucrose as carbon substrate and corn steep liquor as nitrogen source. Enzyme purification studies by ammonium sulphate precipitation and Sephadex G-100 chromatography was evaluated for obtaining purified enzyme. Thermostability of amylase were evaluated with varying concentrations from 0.2 to 0.5 M concentrations of calcium chloride and the highest activity obtained was 3115 U/mL with 0.3 M calcium chloride at 55°C. Effect of temperature and pH on the activity of purified enzyme was evaluated and the purified enzyme showed an activity till 75°C and a pH of 6.5. Application potential of partially purified alpha amylase on desizing of cotton cloth was evaluated with varying enzyme concentrations from 50 to 500 U/mL and the highest desizing activity was found to be at 300 U/mL.
机译：使用我们的实验室分离物黑曲霉（Aspergillus niger sp。）研究了细胞外淀粉酶的产生。在发酵罐中研究了MK 07和工艺变量对酶产生的影响。发现当发酵罐的体积保持在70％，搅拌速率为250 rpm，空气供应量为2.5 vvm，接种物浓度为10％，pH为5.0时，淀粉酶的产量最高。在所有优化条件下，以蔗糖为碳底物，玉米浆为氮源，酶的最高产量为1734 U / mL。通过硫酸铵沉淀和Sephadex G-100色谱法对酶的纯化研究进行了评估，以获得纯化的酶。用浓度范围为0.2至0.5μM的氯化钙评估淀粉酶的热稳定性，在55℃下用0.3μM的氯化钙获得的最高活性为3115μU/ mL。评估温度和pH对纯化的酶的活性的影响，并且纯化的酶显示直至75℃的活性和pH为6.5。用50至500 U / mL的不同酶浓度评估了部分纯化的α淀粉酶在棉布退浆中的应用潜力，发现最高退浆活性为300 U / mL。

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