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Optimization for the Production of Cellulase Enzyme from Municipal Solid Waste Residue by Two Novel Cellulolytic Fungi

机译:用两种新型纤维素分解真菌优化城市生活垃圾中纤维素酶的生产

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摘要

The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus niger and Trichoderma sp.) from municipal solid waste. Municipal solid waste residue (4-5% (w/v)) and peptone and yeast extract (1.0% (w/v)) were found to be the best combination of carbon and nitrogen sources for the production of cellulase by A. niger and Trichoderma sp. Optimum temperature and pH of the medium for the cellulase production by A. niger were 40°C and 6-7, whereas those for the production of cellulase by Trichoderma sp. were 45°C and 6.5. Cellulase production from A. niger and Trichoderma sp. can be an advantage as the enzyme production rate is normally higher as compared to other fungi.
机译:这项研究的主要目的是通过优化生产介质并使用替代碳源(如城市固体废物残渣)来降低纤维素酶的生产成本。在本研究中,我们旨在从城市固体废物中分离出两种新型纤维素酶生产真菌(黑曲霉和木霉菌)。发现市政固体废物残留物(4-5%(w / v))和蛋白and与酵母提取物(1.0%(w / v))是黑曲霉生产纤维素酶的碳源和氮源的最佳组合。和木霉属。黑曲霉生产纤维素酶的培养基的最佳温度和最适pH为40°C和6-7,而木霉属菌株生产纤维素酶的最适温度和pH为6-7。分别为45°C和6.5。黑曲霉和木霉属的纤维素酶生产。由于酶的生产率通常比其他真菌高,因此可以是一个优势。

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