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Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system

机译:波长扫描系统相干反斯托克斯拉曼散射高光谱组织成像

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摘要

We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system for hyperspectral imaging in thick tissue. We use a strategy where the Raman lines are excited sequentially, circumventing the need for a spectrometer. This fibre laser system, consisting of a pump laser synchronized with a rapidly tunable programmable laser (PL), can access Raman lines over a significant fraction of the high wavenumber region (2700–2950 cm−1) at rates of up to 10,000 spectral points per second. To demonstrate its capabilities, we have acquired WS-CARS spectra of several samples as well as images and hyperspectral images (HSI) of thick tissue both in forward and epi-detection. This instrument should be especially useful in providing local biochemical information with surrounding context supplied by imaging.
机译:我们目前在厚组织中的高光谱成像的波长扫描相干反斯托克斯拉曼散射(WS-CARS)光谱系统。我们采用拉曼谱线依次激发的策略,从而避免了对光谱仪的需求。这种光纤激光器系统由与快速可调可编程激光器(PL)同步的泵浦激光器组成,可以在高波数区域(2700–2950 cm -1 )的很大一部分上访问拉曼线速率高达每秒10,000个光谱点。为了证明其功能,我们已经获取了一些样本的WS-CARS光谱以及正向和Epi检测中厚组织的图像和高光谱图像(HSI)。该仪器在通过成像提供周围环境的情况下提供局部生化信息时特别有用。

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