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Imaging cone photoreceptors in three dimensions and in time using ultrahigh resolution optical coherence tomography with adaptive optics

机译：使用具有自适应光学元件的超高分辨率光学相干层析成像技术可在三个维度上及时对锥形感光体进行成像

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Cone photoreceptors in the living human eye have recently been imaged with micron-scale resolution in all three spatial dimensions using adaptive optics optical coherence tomography. While these advances have allowed non-invasive study of the three-dimensional structure of living human cones, studies of their function and physiology are still hampered by the difficulties to monitor the same cells over time. The purpose of this study is to demonstrate the feasibility of cone monitoring using ultrahigh-resolution adaptive optics optical coherence tomography. Critical to this is incorporation of a high speed CMOS camera (125 KHz) and a novel feature-based, image registration/dewarping algorithm for reducing the deleterious effects of eye motion on volume images. Volume movies were acquired on three healthy subjects at retinal eccentricities from 0.5° to 6°. Image registration/dewarping reduced motion artifacts in the movies from 15 μm to 1.3 μm root mean square, the latter sufficient for identifying and tracking cones. Cone row-to-row spacing and outer segment lengths were consistent with that reported in the literature. Cone length analysis demonstrates that UHR-AO-OCT is sufficiently sensitive to measure real length differences between cones in the same 0.5° retinal patch, and requires no more than five measurements of OS length to achieve 95% confidence. We know of no other imaging modality that can monitor foveal or parafoveal cones over time with comparable resolution in all three dimensions.
机译：最近，使用自适应光学光学相干断层扫描技术，已在所有三个空间维度上以微米级的分辨率对人眼中的圆锥形感光体进行了成像。尽管这些进展允许对活体视锥细胞的三维结构进行非侵入性研究，但其功能和生理学研究仍因难以随时间监测相同细胞而受到阻碍。这项研究的目的是证明使用超高分辨率自适应光学光学相干断层扫描技术进行锥体监测的可行性。对此至关重要的是，要结合高速CMOS相机（125 KHz）和新颖的基于特征的图像配准/变形算法，以减少眼睛运动对体积图像的有害影响。在三名健康受试者的视网膜偏心度为0.5°至6°的条件下拍摄了体积电影。图像配准/变形使电影中的运动伪影从15μm减少到1.3μm均方根，后者足以识别和跟踪视锥。锥体的行间距和外部段的长度与文献报道的一致。视锥长度分析表明，UHR-AO-OCT足够灵敏，可以测量同一0.5°视网膜斑块中视锥之间的实际长度差异，并且不超过五次测量OS长度即可达到95％的置信度。我们知道，没有其他影像学方法可以随时间推移在所有三个维度上以可比的分辨率监视中央凹或中央凹圆锥。

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