• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Biochemistry and Biophysics Reports >Binding and structural studies of the complexes of type 1 ribosome inactivating protein from Momordica balsamina with cytosine cytidine and cytidine diphosphate
【2h】

Binding and structural studies of the complexes of type 1 ribosome inactivating protein from Momordica balsamina with cytosine cytidine and cytidine diphosphate

机译：苦瓜中1型核糖体失活蛋白与胞嘧啶胞苷和胞苷二磷酸的复合物的结合和结构研究

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The type 1 ribosome inactivating protein from Momordica balsamina (MbRIP1) has been shown to interact with purine bases, adenine and guanine of RNA/DNA. We report here the binding and structural studies of MbRIP1 with a pyrimidine base, cytosine; cytosine containing nucleoside, cytidine; and cytosine containing nucleotide, cytidine diphosphate. All three compounds bound to MbRIP1 at the active site with dissociation constants of 10−4 M–10−7 M. As reported earlier, in the structure of native MbRIP1, there are 10 water molecules in the substrate binding site. Upon binding of cytosine to MbRIP1, four water molecules were dislodged from the substrate binding site while five water molecules were dislodged when cytidine bound to MbRIP1. Seven water molecules were dislocated when cytidine diphosphate bound to MbRIP1. This showed that cytidine diphosphate occupied a larger space in the substrate binding site enhancing the buried surface area thus making it a relatively better inhibitor of MbRIP1 as compared to cytosine and cytidine. The key residues involved in the recognition of cytosine, cytidine and cytidine diphosphate were Ile71, Glu85, Tyr111 and Arg163. The orientation of cytosine in the cleft is different from that of adenine or guanine indicating a notable difference in the modes of binding of purine and pyrimidine bases. Since adenine containing nucleosidesucleotides are suitable substrates, the cytosine containing nucleosidesucleotides may act as inhibitors.
机译：苦瓜（Morordica balsamina）的1型核糖体失活蛋白（MbRIP1）已显示与RNA / DNA的嘌呤碱基，腺嘌呤和鸟嘌呤相互作用。我们在这里报告与嘧啶碱基胞嘧啶的MbRIP1的结合和结构研究；胞嘧啶核苷，胞苷；和含有胞嘧啶的核苷酸，胞苷二磷酸。这三种化合物都在活性位点以10 −4 M–10 −7 M的解离常数与MbRIP1结合。如前所述，在天然MbRIP1的结构中存在在底物结合位点有10个水分子。在胞嘧啶与MbRIP1结合后，当胞苷与MbRIP1结合时，四个水分子从底物结合位点移出，而五个水分子被移出。当胞苷二磷酸结合到MbRIP1时，七个水分子脱位。这表明胞苷二磷酸在底物结合位点占据了较大的空间，从而增强了掩埋的表面积，因此与胞嘧啶和胞苷相比，它是相对更好的MbRIP1抑制剂。识别胞嘧啶，胞苷和胞苷二磷酸的关键残基为Ile71，Glu85，Tyr111和Arg163。裂隙中胞嘧啶的方向不同于腺嘌呤或鸟嘌呤的方向，表明嘌呤和嘧啶碱基的结合方式存在显着差异。由于含有腺嘌呤的核苷/核苷酸是合适的底物，因此含有胞嘧啶的核苷/核苷酸可以作为抑制剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号