Expression and purification of the full murine NPM2 and study of its interaction with protamines and histones

机译：完整鼠源NPM2的表达和纯化及其与鱼精蛋白和组蛋白相互作用的研究

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摘要

Mouse nucleoplasmin M.NPM2 was recombinantly expressed and the protein consisting of the complete sequence was purified and characterized. Similar to its Xenopus laevis X.NPM2 counterpart, the protein forms stable pentameric complexes and exhibits an almost undistinguishable hydrodynamic ionic strength-dependent unfolding behavior. The interaction of N.PM2 with histones and mouse P1/P2 protamines revealed that these chromosomal proteins bind preferentially to the distal part of the nucleoplasmin pentamer. Moreover, the present work highlights the critical role played by histones H2B and H4 in the association of the histone H2A-H2B dimers and histone octamer with nucleoplasmin.

机译：重组表达小鼠核纤溶酶M.NPM2，并纯化和鉴定由完整序列组成的蛋白质。与其Xenopus laevis X.NPM2对应物相似，该蛋白质形成稳定的五聚体复合物，并表现出几乎不可区分的流体动力学离子强度依赖性展开行为。 N.PM2与组蛋白和小鼠P1 / P2鱼精蛋白的相互作用表明，这些染色体蛋白优先结合至核纤溶酶五聚体的远端。此外，本工作突出了组蛋白H2B和H4在组蛋白H2A-H2B二聚体和组蛋白八聚体与核纤溶酶的结合中所起的关键作用。

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期刊名称 Biochemistry and Biophysics Reports
作者
Katherine Ellard; Jason J. Serpa; Evgeniy V. Petrotchenko; Christoph H. Borchers; Juan Ausió;
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年(卷),期 2016(6),-1
年度 2016
页码 165–171
总页数 7
原文格式 PDF
正文语种
中图分类生物学;
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专利

1. Expression and purification of the full murine NPM2 and study of its interaction with protamines and histones [J] . Katherine Ellard, Jason J. Serpa, Evgeniy V. Petrotchenko, Biochemistry and Biophysics Reports . 2016,第1期

机译：全鼠NPM2的表达和纯化及其与鱼精蛋白和组蛋白的相互作用的研究
2. Expression and purification of the active variant of recombinant murine Golli-interacting protein (GIP)-characterization of its phosphatase activity and interaction with Golli-BG21 [J] . Bamm VV, Harauz G Protein Expression and Purification . 2008,第1期

机译：重组鼠Golli相互作用蛋白（GIP）活性变体的表达和纯化-表征其磷酸酶活性以及与Golli-BG21的相互作用
3. Overexpression, purification, and biochemical and spectroscopic characterization of copper-containing nitrite reductase from Sinorhizobium meliloti 2011. Study of the interaction of the catalytic copper center with nitrite and NO [J] . Ferroni F.M., Guerrero S.A., Rizzi A.C., Journal of Inorganic Biochemistry: An Interdisciplinary Journal . 2012,第Null期

机译：苜蓿中华根瘤菌（Sinorhizobium meliloti）2011年含铜亚硝酸盐还原酶的过表达，纯化以及生化和光谱学表征。催化铜中心与亚硝酸盐和NO的相互作用研究
4. Picosecond laser cross: linking histones to DNA in chromatin: implication in studying histone/DNA interactions [C] . Author(s): D.Angelov Institute of Solid State Physics Sofia Bulgaria, S.Dimitrov Institute of Molecular Biology Sofia Bulgaria, E.Keskinova Institute of Solid State Physics Sofia Bulgaria, Intermational conference on laser applications in life sciences . 1994

机译：PicoSecond激光十字：将组蛋白联系在染色质中DNA：在研究组蛋白/ DNA相互作用时意味着
5. Expression of histone deacetylase enzymes in murine and chick optic nerve. [D] . Tiwari, Sarika. 2013

机译：组蛋白脱乙酰基酶在鼠和小鸡视神经中的表达。
6. Crystal structure and function of human nucleoplasmin (Npm2): a histone chaperone in oocytes and embryos [O] . Olga Platonova, Ildikó V. Akey, James F. Head, -1

机译：晶体结构和人nucleoplasmin（Npm2）的功能：在卵母细胞和胚胎组蛋白分子伴侣
7. Expression and purification of the full murine NPM2 and study of its interaction with protamines and histones [O] . Ellard Katherine, Serpa Jason J., Petrotchenko Evgeniy V., 2016

机译：全鼠NPM2的表达和纯化及其与鱼精蛋白和组蛋白相互作用的研究

Expression and purification of the full murine NPM2 and study of its interaction with protamines and histones

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