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首页> 美国卫生研究院文献>Biochemical Journal >Methods of automatic nucleotide-sequence analysis. Multicomponent spectrophotometric analysis of mixtures of nucleic acid components by a least-squares procedure
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Methods of automatic nucleotide-sequence analysis. Multicomponent spectrophotometric analysis of mixtures of nucleic acid components by a least-squares procedure

机译：自动核苷酸序列分析的方法。最小二乘程序对核酸组分混合物进行多组分分光光度分析

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1. A theoretical analysis of the errors in multicomponent spectrophotometric analysis of nucleoside mixtures, by a least-squares procedure, has been made to obtain an expression for the error coefficient, relating the error in calculated concentration to the error in extinction measurements. 2. The error coefficients, which depend only on the `library' of spectra used to fit the experimental curves, have been computed for a number of `libraries' containing the following nucleosides found in s-RNA: adenosine, guanosine, cytidine, uridine, 5-ribosyluracil, 7-methylguanosine, 6-dimethylaminopurine riboside, 6-methylaminopurine riboside and thymine riboside. 3. The error coefficients have been used to determine the best conditions for maximum accuracy in the determination of the compositions of nucleoside mixtures. 4. Experimental determinations of the compositions of nucleoside mixtures have been made and the errors found to be consistent with those predicted by the theoretical analysis. 5. It has been demonstrated that, with certain precautions, the multicomponent spectrophotometric method described is suitable as a basis for automatic nucleotide-composition analysis of oligonucleotides containing nine nucleotides. Used in conjunction with continuous chromatography and flow chemical techniques, this method can be applied to the study of the sequence of s-RNA.
机译：1.通过最小二乘程序对核苷混合物的多组分分光光度法分析中的误差进行了理论分析，以获得误差系数的表达式，将计算浓度的误差与消光测量中的误差相关联。 2.对于包含在s-RNA中发现的以下核苷的许多“文库”，已计算出误差系数（仅取决于用于拟合实验曲线的光谱的“文库”）：腺苷，鸟苷，胞苷，尿苷，5-核糖基尿嘧啶，7-甲基鸟苷，6-二甲基氨基嘌呤核苷，6-甲基氨基嘌呤核苷和胸腺嘧啶核苷。 3.误差系数已被用于确定确定核苷混合物组成的最大准确性的最佳条件。 4.已经对核苷混合物的组成进行了实验测定，发现误差与理论分析所预测的误差一致。 5.已经证明，在某些预防措施的情况下，所描述的多组分分光光度法适合作为自动对包含九个核苷酸的寡核苷酸进行核苷酸组成分析的基础。与连续色谱和流化学技术结合使用，该方法可用于研究s-RNA的序列。

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