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首页> 美国卫生研究院文献>Biochemical Journal >Oxygen- or organic hydroperoxide-induced chemiluminescence of brain and liver homogenates.
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Oxygen- or organic hydroperoxide-induced chemiluminescence of brain and liver homogenates.

机译：氧或有机氢过氧化物诱导的脑和肝匀浆的化学发光。

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Oxygenation of anaerobically isolated brain and liver homogenates is associated with chemiluminescence and formation of lipid hydroperoxides, the latter determined by the thiobarbituric acid assay. Light emission and formation of malonaldehyde are 20-fold higher in the brain than in liver; chemiluminescence of both decays when accumulation of malonaldehyde ceases. Exogenous organic peroxides, such as t-butyl hydroperoxide, inhibit the light-emission response to oxygenation by brain homogenate, whereas they enhance that of liver homogenate. t-Butyl hydroperoxide-induced photoemission of liver homogenate shows a polyphasic kinetic pattern that is O2-dependent. The spectral analysis of chemiluminescence arising from brain and liver homogenates on oxygenation shows a spectrum with five emission bands at 420-450, 475-485, 510-540, 560-580 and 625-640 nm. These bands are subjected to intensity changes or shifts of the wavelength whenever t-butyl hydroperoxide is present, either inhibiting or stimulating light emission. The blue-band chemiluminescence, around 435 nm, is possibly due to the weak light emission arising from excited carbonyl compounds [Lloyd (1965) J. Chem. Soc. Faraday Trans. 61, 2182-2193; Vassil'ev (1965) Opt. Spectrosc. (USSR) 18, 131-135], whereas the presence of other bands suggests generation of singlet molecular oxygen either in the process triggered on oxygenation (lipid oxygenation) or after supplementation with organic hydroperoxides. We offer several explanations for the spectral analysis presented here.
机译：厌氧分离的大脑和肝脏匀浆的氧合与化学发光和脂质氢过氧化物的形成有关，后者由硫代巴比妥酸试验确定。大脑中的发光和丙二醛的形成比肝脏高20倍；当丙二醛的积累停止时，两者的化学发光都会减弱。外源有机过氧化物，例如氢过氧化叔丁基，可抑制脑匀浆对氧合的发光反应，而它们却能增强肝匀浆的光响应。叔丁基过氧化氢诱导的肝匀浆的光发射显示出依赖于O2的多相动力学模式。由大脑和肝脏匀浆物在氧合作用下产生的化学发光光谱分析显示，在420-450、475-485、510-540、560-580和625-640 nm处具有五个发射带。每当存在叔丁基氢过氧化物时，这些谱带就会发生强度变化或波长变化，从而抑制或刺激光发射。约435 nm的蓝带化学发光可能是由于激发的羰基化合物产生的弱发光[Lloyd（1965）J. Chem。 Soc。法拉第反式61，2182-2193; Vassil'ev（1965）选项。光谱。 （USSR）18，131-135]，而其他条带的存在则表明在氧合（脂质氧合）触发的过程中或补充有机氢过氧化物后产生单线态分子氧。我们为此处介绍的光谱分析提供了几种解释。

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