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Effects of preincubation of primary monolayer cultures of rat hepatocytes with low- and high-density lipoproteins on the subsequent binding and metabolism of human low-density lipoprotein.

机译：低密度脂蛋白和高密度脂蛋白对大鼠肝细胞原代单层培养物的预孵育对人低密度脂蛋白随后结合和代谢的影响。

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1. There are two distinct binding sites (Site 1 and Site 2) for human low-density lipoprotein (LDL) on rat hepatocytes in monolayer culture [Salter, Saxton & Brindley (1986) Biochem. J. 240, 549-557]. 2. Binding of 125I-LDL to Site 1, but not to Site 2, is up-regulated between 20 and 44 h in culture by preincubation of the cells with human high-density lipoprotein 3 (HDL3). 3. A similar preincubation with HDL2 had no significant effect on binding to either site. 4. Preincubation with human LDL led to a partial down-regulation of subsequent binding of 125I-LDL to Site 1. Since binding after incubation with LDL was measured at 37 degrees C, binding to Site 2 could not be distinguished from LDL that had been internalized by the cells. 5. Hepatocytes were shown to degrade 125I-LDL, resulting in the accumulation of [125I]iodotyrosine in the medium. Evidence was found that iodotyrosine may be further degraded by deiodinase produced by the cells. 6. Regulation of binding to Site 1 by preincubation with LDL or HDL3 was found to lead to a parallel regulation of LDL degradation. 7. It is concluded that rat hepatocytes not only bind but also metabolize human LDL and that these processes are under metabolic regulation.

机译：1.在单层培养中，大鼠肝细胞上的人类低密度脂蛋白（LDL）有两个不同的结合位点（位点1和位点2）[Salter，Saxton＆Brindley（1986）Biochem。Chem。，第2期，第1期。 J. 240，549-557]。 2.通过将细胞与人高密度脂蛋白3（HDL3）预孵育，可在培养20到44 h之间将125I-LDL与位点1的结合而不是位点2的结合上调。 3.与HDL2的类似预温育对结合至任一位点均无明显影响。 4.与人LDL的预孵育导致125I-LDL与位点1的后续结合的部分下调。由于在37°C下测量了与LDL孵育后的结合，因此无法区分与位点2的结合。被细胞内在化。 5.显示肝细胞降解125I-LDL，导致[125I]碘酪氨酸在培养基中积累。有证据表明，碘酪氨酸可能会被细胞产生的脱碘酶进一步降解。 6.发现通过与LDL或HDL3预孵育来调节与位点1的结合导致平行调节LDL降解。 7.得出的结论是，大鼠肝细胞不仅会结合而且会代谢人LDL，并且这些过程处于代谢调节之下。

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期刊名称 Biochemical Journal
作者
A M Salter; M Bugaut; J Saxton; S C Fisher; D N Brindley;
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年(卷),期 1987(247),1
年度 1987
页码 79–84
总页数 6
原文格式 PDF
正文语种
中图分类分子生物学;
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专利

1. Effects of preincubation of primary monolayer cultures of rat hepatocytes with low- and high-density lipoproteins on the subsequent binding and metabolism of human low-density lipoprotein [J] . A M Salter, D N Brindley, J Saxton, The biochemical journal . 1987,第1期

机译：低密度脂蛋白和高密度脂蛋白对大鼠肝细胞原代单层培养物的预孵育对人低密度脂蛋白随后结合和代谢的影响
2. Characterization of the binding of human low-density lipoprotein to primary monolayer cultures of rat hepatocytes [J] . A M Salter, D N Brindley, J Saxton The biochemical journal . 1986,第2期

机译：人低密度脂蛋白与大鼠肝细胞原代单层培养物结合的特征
3. Adenoviral expression of human lecithin-cholesterol acyltransferase in nonhuman primates leads to an antiatherogenic lipoprotein phenotype by increasing high-density lipoprotein and lowering low-density lipoprotein. [J] . Amar MJ, Shamburek RD, Vaisman B Metabolism: Clinical and Experimental . 2009,第4期

机译：人卵磷脂胆固醇酰基转移酶在非人灵长类动物中的腺病毒表达通过增加高密度脂蛋白和降低低密度脂蛋白而导致抗动脉粥样硬化脂蛋白表型。
4. Predicting Pathways for Metabolism of Primaquine by Primary Human Hepatocytes Using UHPLC-QToF-MS With ~(13)C Stable Isotope Labeling [C] . Bharathi Avula, Babu L. Tekwani, Narayan D. Chaurasiya, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：用UHPLC-QTOF-MS预测原发性人肝细胞代谢的途径，用〜（13）C稳定同位素标记
5. Signaling mechanisms regulating activation of human endothelial cells by oxidized phospholipids and their modulation by high-density lipoprotein. [D] . Gharavi, Nima Mousaviol. 2006

机译：信号传导机制通过氧化磷脂调节人类内皮细胞的活化，并通过高密度脂蛋白对其进行调节。
6. Characterization of the binding of human low-density lipoprotein to primary monolayer cultures of rat hepatocytes. [O] . A M Salter, J Saxton, D N Brindley 1986

机译：人低密度脂蛋白与大鼠肝细胞原代单层培养物结合的特征。
7. Effects of preincubation of primary monolayer cultures of rat hepatocytes with low- and high-density lipoproteins on the subsequent binding and metabolism of human low-density lipoprotein. [O] . Salter, A M, Bugaut, M, Saxton, J, 1987

机译：低密度脂蛋白和高密度脂蛋白对大鼠肝细胞原代单层培养物的预孵育对人低密度脂蛋白随后结合和代谢的影响。
8. Norepinephrine Decreases EGF (Epidermal Growth Factor) Binding in Primary Rat Hepatocyte Cultures [R] . Cruise, J. L. , Cotecchia, S. , Michalopoulos, G. 1986

机译：去甲肾上腺素降低原代大鼠肝细胞培养物中EGF（表皮生长因子）的结合

Effects of preincubation of primary monolayer cultures of rat hepatocytes with low- and high-density lipoproteins on the subsequent binding and metabolism of human low-density lipoprotein.

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