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首页> 美国卫生研究院文献>Biochemical Journal >Evidence for ‘lock and key’ character in an anti-phosphonate hydrolytic antibody catalytic site augmented by non-reaction centre recognition: variation in substrate selectivity between an anti-phosphonate antibody an anti-phosphate antibody and two hydrolytic enzymes
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Evidence for ‘lock and key’ character in an anti-phosphonate hydrolytic antibody catalytic site augmented by non-reaction centre recognition: variation in substrate selectivity between an anti-phosphonate antibody an anti-phosphate antibody and two hydrolytic enzymes

机译：通过非反应中心识别增强抗磷酸水解抗体催化位点锁定和关键特征的证据：抗磷酸酯抗体抗磷酸抗体和两种水解酶之间的底物选择性变化

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摘要

The substrate selectivities of an anti-phosphonate and an anti-phosphate kinetically homogeneous polyclonal catalytic antibody preparation and two hydrolytic enzymes were compared by using hapten-analogous and truncated carbonate and ester substrates each containing a 4-nitrophenolate leaving group. Syntheses of the truncated substrates devoid of recognition features in the non-leaving group parts of the substrates are reported. The relatively high kinetic selectivity of the more active anti-phosphonate antibody preparation is considered to depend on a relatively rigid catalytic site with substantial reaction centre specificity together with other important recognition interactions with the extended non-leaving group part of the substrate. In contrast, the less catalytically active, more flexible anti-phosphate antibody exhibits much lower kinetic selectivity for the substrate reaction centre comparable with that of the hydrolytic enzymes with activity much less dependent on recognition interactions with the non-leaving group part of the substrate. The ways in which haptenic flexibility and IgG architecture might contribute to the differential kinetic selectivities are indicated.
机译：通过使用均含4-硝基酚盐离去基团的半抗原类似物和截短的碳酸盐和酯底物，比较了抗膦酸盐和抗磷酸盐动力学均一的多克隆催化抗体制剂以及两种水解酶的底物选择性。报道了在基底的非离开基团部分中缺乏识别特征的截短的基底的合成。更具活性的抗膦酸酯抗体制剂的相对较高的动力学选择性被认为取决于具有基本反应中心特异性的相对刚性的催化位点，以及与底物的延伸的非离去基团部分的其他重要的识别相互作用。相反，催化活性较低，更具柔性的抗磷酸盐抗体与水解酶相比，对底物反应中心表现出低得多的动力学选择性，而其活性与依赖于与底物非离去基团部分的识别相互作用的依赖性更小。指出了半抗原的柔性和IgG结构可能有助于差异动力学选择性的方式。

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