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首页> 美国卫生研究院文献>The Journal of Neuroscience >Schwann Cell But Not Olfactory Ensheathing Glia Transplants Improve Hindlimb Locomotor Performance in the Moderately Contused Adult Rat Thoracic Spinal Cord
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Schwann Cell But Not Olfactory Ensheathing Glia Transplants Improve Hindlimb Locomotor Performance in the Moderately Contused Adult Rat Thoracic Spinal Cord

机译：雪旺氏细胞但不嗅鞘胶质细胞移植可改善中度挫伤成年大鼠胸脊髓的后肢运动能力

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摘要

Cultured adult rat Schwann cells (SCs) or olfactory ensheathing glia (OEG), or both, were transplanted in the adult Fischer rat thoracic (T9) spinal cord 1 week after a moderate contusion (10 gm, 12.5 mm, NYU impactor). Rats received either a total of 2 × 106 cells suspended in culture medium or culture medium only (controls). At 12 weeks after injury, all grafted animals exhibited diminished cavitation. Although in medium-injected rats 33% of spinal tissue within a 5-mm-long segment of cord centered at the injury site was spared, significantly more tissue was spared in SC (51%), OEG (43%), and SC/OEG (44%) grafted animals. All three types of glial grafts were filled with axons, primarily of spinal origin. SC grafts contained more myelinated axons than SC/OEG and OEG grafts. Both types of SC-containing grafts expressed more intense staining for glial fibrillary acidic protein and chondroitin sulfate proteoglycan compared with OEG-only grafts. Retrograde tracing demonstrated that the number of propriospinal and brainstem axons reaching 5–6 mm beyond the grafted area was significantly higher with SC and SC/OEG grafts but not with OEG-only grafts compared with controls. Corticospinal fibers terminated closer to the lesion epicenter in all grafted animals than in controls. With SC-only grafts, a modest but statistically significant improvement in hindlimb locomotor performance was detected at 8–11 weeks after injury. Thus, in addition to this functional improvement, our results show that an SC graft is more effective in promoting axonal sparing/regeneration than an SC/OEG or OEG graft in the moderately contused adult rat thoracic spinal cord.
机译：培养的成年大鼠雪旺氏细胞（SCs）或嗅鞘神经胶质细胞（OEG）或两者均在中度挫伤（10 gm，12.5 mm，NYU撞击器）一周后移植到成年Fischer大鼠胸（T9）脊髓中。大鼠总共接受了2×10 6 细胞悬浮于培养基中或仅悬浮于培养基中（对照）。受伤后第12周，所有移植动物的气穴现象均减少。尽管在中等剂量注射的大鼠中，以损伤部位为中心的5毫米长的脊髓节段中有33％的脊髓组织得到了保留，但SC（51％），OEG（43％）和SC / OEG（44％）移植了动物。所有三种类型的神经胶质移植物都充满了主要来自脊柱的轴突。 SC移植物比SC / OEG和OEG移植物包含更多的髓鞘轴突。与仅使用OEG的移植物相比，这两种类型的包含SC的移植物对神经胶质纤维酸性蛋白和硫酸软骨素蛋白聚糖的染色都更强烈。逆行示踪表明，与对照组相比，SC和SC / OEG移植物的脊椎和脑干轴突的数量明显超出移植面积5–6 mm，而仅OEG的移植物则没有。与所有对照组相比，所有移植动物的皮质脊髓纤维均终止于病灶中心。对于仅SC移植物，在受伤后8-11周发现后肢运动能力有适度但有统计学意义的改善。因此，除了这种功能上的改善外，我们的结果表明，在中度挫伤的成年大鼠胸脊髓中，SC移植物比SC / OEG或OEG移植物更有效地促进轴突保留/再生。

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