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首页> 外文期刊>Glia >Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery
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Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery

机译：雪旺细胞和/或嗅鞘神经胶质细胞移植到挫伤的脊髓中：存活，迁移，轴突缔合和功能恢复

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Schwann cells (SCs) and olfactory ensheathing glia (OEG) have shown promise for spinal cord injury repair. We sought their in vivo identification following transplantation into the contused adult rat spinal cord at 1 week post-injury by: (i) DNA in situ hybridization (ISH) with a Y-chromosome specific probe to identify male transplants in female rats and (ii) lentiviral vector-mediated expression of EGFP. Survival, migration, and axon-glia association were quantified from 3 days to 9 weeks post-transplantation. At 3 weeks after transplantation into the lesion, a 60-90% loss of grafted cells was observed. OEG-only grafts survived very poorly within the lesion (<5%); injection outside the lesion led to a 60% survival rate, implying that the injury milieu was hostile to transplanted cells and or prevented their proliferation. At later times post-grafting, p75+/EGFP- cells in the lesion outnumbered EGFP+ cells in all paradigms, evidence of significant host SC infiltration. SCs and OEG injected into the injury failed to migrate from the lesion. Injection of OEG outside of the injury resulted in their migration into the SC-injected injury site, not via normal-appearing host tissue but along the pia or via the central canal. In all paradigms, host axons were seen in association with or ensheathed by transplanted glia. Numerous myelinated axons were found within regions of grafted SCs but not OEG. The current study details the temporal survival, migration, axon association of SCs and OEG, and functional recovery after grafting into the contused spinal cord, research previously complicated due to a lack of quality, long-term markers for cell tracking in vivo.
机译：雪旺细胞（SCs）和嗅鞘神经胶质细胞（OEG）已显示有望修复脊髓损伤。我们寻求在损伤后1周移植到挫伤的成年大鼠脊髓后的体内识别方法：（i）用Y染色体特异性探针进行DNA原位杂交（ISH）以鉴定雌性大鼠中的雄性移植物，以及（ii慢病毒载体介导的EGFP表达。移植后3天至9周对存活，迁移和轴突-胶质细胞结合进行了定量。移植到病变部位后3周，观察到移植细胞损失60-90％。仅OEG的移植物在病灶内的存活率非常低（<5％）。在病灶外注射可导致60％的存活率，这表明损伤环境对移植的细胞不利，或阻止了它们的增殖。移植后晚些时候，病变中的p75 + / EGFP-细胞在所有范式中都超过了EGFP +细胞，这表明宿主SC有明显的浸润。注入损伤的SC和OEG未能从病变中迁移。在伤口外注射OEG导致它们迁移到SC注射的损伤部位，而不是通过正常出现的宿主组织，而是沿着小脑或通过中央管。在所有范例中，都可以看到宿主轴突与移植的神经胶质细胞相关或被其包裹。在移植的SC区域内发现了许多有髓的轴突，但在OEG中却没有。目前的研究详述了暂时存活，迁移，SCs和OEG的轴突结合以及移植到挫伤的脊髓后的功能恢复，由于缺乏用于体内细胞追踪的高质量长期标记物，以前的研究非常复杂。

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