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首页> 美国卫生研究院文献>Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology >Detection of Babesia infection among human goats and sheep using microscopic and molecular methods in the city of Kuhdasht in Lorestan Province West of Iran
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Detection of Babesia infection among human goats and sheep using microscopic and molecular methods in the city of Kuhdasht in Lorestan Province West of Iran

机译：使用显微镜和分子方法在伊朗西部洛雷斯坦省库赫达什特市检测人山羊和绵羊的巴贝虫感染

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Babesiosis is a lethal protozoan disease, responsible for the loss of livestock in Iran and in the world. The purpose of the current study was to detect and identify Babesia spp. infection using microscopic and molecular methods in human, sheep and goats in Kuhdasht region, in the Lorestan Province, west of Iran. During 2013, a total of 384 blood smear samples were collected from 51 goats, 306 sheep suspected of Babesiosis infection and 27 humans from Kuhdasht region. The blood samples were fixed, stained and under light microscopic examined. DNA samples were extracted and amplified by polymerase chain reaction of 18S-rRNA gene. PCR and the semi-nested PCR were performed to identify to Babesi spp. and to differentiate genus of Theileria and Babesia spp. The results of microscopic examination indicated that a total of 47 (12.2%) samples were positive for Babesia spp. infection: 38 (9.9%) belonging to sheep and 9 to goats (2.3%). No Babesia was observed in human samples. The PCR showed a band size of 389 bp, of Babesia spp. and the semi-nested PCR detected B. ovis with a band size of 186 bp. By molecular method, 16 (4.2%) sheep and 2 (0.5%) goat blood samples were infected by Babesia. Totally, 18 samples (4.7%) were observed to have Babesia, while no infection was found in human. Thus, the results of our study have shown sheep and goats could be vulnerable to Babesia spp., especially B. ovis in Lorestan Province, Iran. Therefore, studies on the status of the animal Piroplasmosis especially Theileriosis are recommended.
机译：婴儿病是一种致死性的原生动物疾病，是伊朗和全世界牲畜损失的原因。本研究的目的是检测和鉴定巴氏杆菌属。使用显微镜和分子方法在伊朗西部洛雷斯坦省库赫达斯特地区的人类，绵羊和山羊身上感染。 2013年期间，从Kuhdasht地区的51只山羊，306只怀疑是巴贝虫病感染的绵羊和27位人类收集了384份血液涂片样本。固定血样，染色并在光学显微镜下检查。通过18S-rRNA基因的聚合酶链反应提取DNA样品并进行扩增。进行PCR和半巢式PCR以鉴定Babesi spp。并区分泰勒虫和巴贝斯虫属。显微镜检查的结果表明，总共有47个样品（12.2％）为巴氏杆菌属阳性。感染：38（9.9％）属于绵羊，9属于山羊（2.3％）。在人类样本中未观察到贝贝虫。 PCR显示带状杆菌（Babesia spp）389 bp。半巢式PCR检测到带B.ovis的条带为186bp。通过分子方法，巴贝斯虫感染了16（4.2％）绵羊和2（0.5％）山羊血样。总共观察到18个样本（4.7％）患有巴贝虫病，而在人类中未发现感染。因此，我们的研究结果表明，绵羊和山羊可能易患巴贝斯虫，尤其是伊朗洛雷斯坦省的牛卵杆菌。因此，建议对动物胞浆菌病特别是泰勒虫病的状况进行研究。

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